CD123^+CD11c^-pDC在初发类风湿关节炎患者外周血及关节液中的表达  

The expression of the peripheral blood and synovial fluid CD123^+CD11c^-pDC in the onset patients with rheumatoid arthritis

作　　者：罗莉[1] 胡永玮[2] 刘盼盼[2] 吉鹏[2] 杜文静[2] 

机构地区：[1]新疆医科大学第一附属医院风湿科,乌鲁木齐830054 [2]新疆医科大学,乌鲁木齐830054

出　　处：《中国免疫学杂志》2013年第8期844-847,共4页Chinese Journal of Immunology

基　　金：自治区高校科研计划项目(XJEDU2010S26);国家大学生创新实验计划项目(CX2012026)

摘　　要：目的:探讨外周血及关节液中浆细胞样树突状细胞(Plasmaetoid dendritic cell,pDC)的数量在初发类风湿关节炎(RA)患者中的表达,进一步明确pDC在类风湿关节炎发病机制中的作用。方法:采集RA组患者(30人),骨关节炎(OA)组(25人),健康对照组(25人)抗凝外周血及关节液,流式细胞术检测CD123+CD11c-pDC的表型及数量。结果:活动性RA患者外周血中CD123+CD11c-pDC(1.69%±0.97%)与OA组(5.38%±2.31%)相比含量减少,差异具有统计学意义(P<0.05),与健康对照组(5.63%±2.33%)相比含量减少,差异具有统计学意义(P<0.05),后两者相比差异无统计学意义(P>0.05)。关节液中两组CD123+CD11c-pDC含量差异无统计学意义(P>0.05),pDC与DAS28评分及疾病的活动度指标RF、ESR、CRP无相关性。结论:初发RA患者体内外周血中CD123+CD11c-pDC含量减少与机体免疫功能紊乱有关,这对进一步阐明RA的发病机制及探索新的靶向治疗起到一定的积极作用。Objective：To investigate the number of plasmaetoid dendritic cell in the peripheral blood and synovial fluid of patients with new-onset and active rheumatoid arthritis（RA）,to further clarify the role in the pathogenesis of RA.Methods： Collected the anticoagulant peripheral blood and Synovial fluid of RA patient（n=30）,Osteoarthritis group（n=25） and health control group（n=25）,and detected the percentage of CD123＋CD11c-pDC cell by flow cytometry.Results： The number of CD123＋CD11c-pDC cell in RA PB（1.69%±0.97%） were lower than that in OA group（5.38%±2.31%）（P〈0.05） and health group（5.63%±2.33%）（P〈0.05）,the latter two showed no difference.Those cells showed no difference in OA SF and RA SF Statistically.pDC and DAS28 score or disease activity index RF,ESR and CRP were no correlation.Conclusion： The decrease of CD123＋CD11c-pDC in RA PB,the existence of that in RA SF promted the development of the disease.That played a positive role of elucidating the pathogenesis of RA and exploring new targeted treatment.

关 键 词：PDC 类风湿关节炎 疾病活动度 CD123+CD11c- 

分 类 号：R392.12[医药卫生—免疫学]