吉林省汉族154例正常人HLA-Cw及KIR基因型频率的分析  

Analysis of the expression of HLA-Cw and its corresponding stimulatory and inhibitory KIR in 154 cases of Jilin Han Population

作　　者：韩瑜[1] 焦立新[1] 马宁[2] 赵令[2] 姜振宇[2] 

机构地区：[1]长春市中心血站,长春130031 [2]吉林大学第一医院风湿病科,长春130021

出　　处：《中国免疫学杂志》2013年第8期853-856,865,共5页Chinese Journal of Immunology

摘　　要：目的:了解中国人HLA-Cw与KIR的识别方式,为今后研究其在疾病中的作用提供理论基础。方法:对154例吉林汉族无偿献血者,以PCR-SSP技术进行KIR和HLA-Cw基因分型。根据HLA-Cw与KIR识别方式,分析个体HLA-Cw与相应激活性或抑制性KIR受体的识别情况。结果:吉林汉族人群中,HLA-C2Lys80+2DL1的组合频率最高为27.27%,其次分别HLA-C1Asn80+2DL2/2DL3为68.83%,2DS2+HLA-C1Asn80为9.74%,2DS1+HLA-C2Lys80为9.74%,分别有72.08%和21.43%的观察对象表达KIR2DL1、KIR2DS1而无相应HLA-Cw表达;21.43%的个体表达KIR2DS1而不表达HLA-Cwlys-KIR2DL1配对。2.60%的个体表达KIR2DS2而不表达HLA-Cwasn-KIR2DL2/L3配对。结论:在吉林汉族人群中,抑制性HLA-Cw-KIR受配体对表达高于活化性受配体对,约20%的个体单独表达激活性HLA-Cw-KIR受配体对。Objective：To investigate the distribution of HLA-Cw-KIR in Jilin Han population,provided a theoretical basis for future studies of its role in the disease.Methods： 154 cases of Jilin Han unpaid blood donors,KIR and HLA-Cw genotyping by PCR-SSP.HLA-Cw KIR means of identification,analysis of individual HLA-Cw and the corresponding activation or inhibitory KIR receptor recognition.Results： In this study,the expression frequency of HLA-C2Lys80＋2DL1 was 27.27%,HLA-C1Asn80＋2DL2/2DL3 was 68.83%,2DS2＋HLA-C1Asn80 was 9.74%,2DS1＋HLA-C2Lys80 was 9.74%.There were 72.08%of pepole only express KIR2DL1 without expression of HLA-Cw,21.43% only express KIR2DS1 without expression of HLA-Cwlys-KIR2DL1,2.60% only express KIR2DS2 without expression of HLA-Cwasn-KIR2DL2/L3.Conclusion： In Jilin Han population,inhibition of activated paired nature of HLA-Cw and-of KIR pairs was higher than about 20 percent of the individual separate expression of activated HLA-Cw and-of KIR matching.

关 键 词：HLA-CW 杀伤免疫球蛋白样受体 配体 吉林汉族 

分 类 号：R392.1[医药卫生—免疫学]