东祁连山线叶嵩草内生细菌X4的产吲哚乙酸、解磷、抗菌和耐盐特性研究及分子鉴定  

Identification and determination of biological characteristics of Kobresia capillifolia endophytic bacteria X4 in the East Qilian Mountain Alpine grasslands

作　　者：高晓星[1] 满百膺[1] 陈秀蓉[1] 杨成德[1] 

机构地区：[1]甘肃农业大学草业学院草业生态系统教育部重点实验室甘肃省草业工程实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草业学报》2013年第4期137-146,共10页Acta Prataculturae Sinica

基　　金：甘肃省科技支撑计划(090NKCA082)资助

摘　　要：线叶嵩草内生细菌X4分离自东祁连山高寒草地,对其产吲哚乙酸、解磷、抗菌和耐盐特性及其分子生物学特性研究结果表明,其在含色氨酸的金氏培养基中分泌吲哚乙酸的量为50.74mg/L,溶磷圈直径/菌落直径(D/d)值分别为3.23和4.31,对辣椒立枯丝核病菌、黄瓜枯萎病菌、油菜菌核病菌及番茄灰霉病菌都有抑制作用,且致菌丝生长畸形,菌落生长的酸碱度范围为7～10,耐NaCl溶液范围为<7%,菌体短杆状,革兰氏阴性,无芽孢,16SrDNA序列与普城沙雷氏菌(Serratia plymuthica)最为接近。该菌具有微生物杀菌剂、改良土壤等潜力。The characteristics of an endophytic bacterium （X4）, isolated from leaves of Kobresia capilliJblia in the East Qilian Mountains Alpine grasslands, were studied using Salkowski colorimetric analysis, the phos phorus cycle and confronting cultivation. Secretion of IAA was 50.74 mg/L in King＇s medium containing tryp tophan. Its dissolved phosphorus circle diameter/bacterial colony diameter （D/d） values were 4.31 and 3.23 respectively. The bacteria inhibited pathogenic fungi, such as Rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, and Fusarium oacysporum, and led to abnormal mycelial growth of these fungi. The pH range of colony growth was 5-10 and resistance to NaC1 solution was less than 7%. Thalli were short rods, and Gram-negative with no spores. The 16S rDNA gene sequence of X4 was most similar to Serratia plymutkica. The bacterium has the potential to make microbial fungicides and to improve soil.

关 键 词：内生细菌 普城沙雷氏菌 生物学 16S rDNA鉴定 

分 类 号：S182[农业科学—农业基础科学]