柱花草磷转运蛋白SgPT1的克隆和表达分析  

Cloning and analysis of the phosphate transporter protein SgPT1 from Stylosanthes

作　　者：孙丽莉[1,2,3] 陈志坚[3] 刘攀道[1,2,3] 廖红[3] 刘国道[1,2] 田江[3] 

机构地区：[1]海南大学农学院 [2]中国热带农业科学院品种资源研究所,海南儋州571737 [3]华南农业大学亚热带农业生物资源保护与利用国家重点实验室根系生物学研究中心,广东广州510642

出　　处：《草业学报》2013年第4期187-196,共10页Acta Prataculturae Sinica

基　　金：国家自然科学基金(31025022);国家重点基础研究973项目(2007CB108903);中国农业产业体系专项基金(CARS-35)资助

摘　　要：磷是植物生长所必需的大量元素。高亲和磷转运子是控制植物对磷吸收和转运的主要蛋白。本研究以磷高效的柱花草基因型TPRC2001-1为对象,首先建立低磷胁迫下TPRC2001-1根系全长cDNA文库。通过同源克隆的方法,首次克隆到编码柱花草高亲和磷转运蛋白的基因,SgPT1。该基因全长cDNA为1 994bp,编码538个氨基酸残基,蛋白分子量为59kD。SgPT1蛋白具有高亲和磷转运子的结构特点,即含有"6+6"跨膜结构。而且,定量PCR分析结果表明低磷胁迫显著促进SgPT1在根部的表达,表明该基因可能参与低磷胁迫下磷的吸收和转运的过程。总之,以上结果表明SgPT1的加强表达可能是TPRC2001-1适应低磷胁迫的分子机制之一,为进一步研究柱花草适应低磷的分子机制提供候选基因。Phosphorous （P） is one of the essential macronutrients and a high-affinity phosphate transporter （PT） plays an important role in P absorption and transport. SgPT1 encodes a high-affinity phosphate trans porter from the roots of TPRC2001-1 which is a P efficient genotype. It was homologously cloned and a full length cDNA library was established under phosphorous deficiency conditions. The full length of SgPT1 is 1 994 bp, and it encodes 538 amino acids. The protein molecular weight of SgPT1 is 59 kD. The protein sec ondary structure is a 6 ＋6 configuration, which is consistent with most high-affinity PT. The expression of SgPT1 was strongly enhanced by low phosphorous （LP） stress in stylo TPRC2001-1 root indicating that SR PT1 potentially participated in P uptake and transport under LP conditions. The increased expression of SgPT1 was one of the molecular mechanisms for style adaptation to LP stress and SgPT1 might be one of the candi date genes for clarifying the mechanisms.

关 键 词：柱花草 磷 磷转运子 同源克隆 SgPT1 

分 类 号：S543.9[农业科学—作物学]