VITEK 2C检测鲍曼不动杆菌对阿米卡星体外敏感性的性能评价  

Evaluation on the performance of VITEK 2C for the susceptibility of Acinetobacter baumannii to amikacin

作　　者：黄晓春[1] 陈岩[1] 李园[1] 许育[1] 秦阳华[1] 

机构地区：[1]第二军医大学附属长海医院实验诊断科,上海200433

出　　处：《检验医学》2013年第8期666-670,共5页Laboratory Medicine

摘　　要：目的评价VITEK 2COMPACT全自动细菌鉴定仪(简称VITEK 2C)和AST-GN13卡检测鲍曼不动杆菌对阿米卡星体外敏感性的性能。方法采用微量肉汤稀释(BMD)法、Kirby-Bauer纸片扩散(KB)法和VITEK 2C分别检测38株临床分离的鲍曼不动杆菌对阿米卡星的敏感性,以BMD法为参比方法,评价VITEK 2C和KB法的性能。通过多重聚合酶链反应(PCR)扩增鲍曼不动杆菌氨基糖苷修饰酶基因以研究其耐药基因型。结果以BMD法为参比方法,VITEK 2C和AST-GN13卡检测鲍曼不动杆菌对阿米卡星的敏感性时19株菌株(50.0%)出现重大误差,2株(5.3%)出现次要误差。多重PCR结果也显示鲍曼不动杆菌氨基糖苷修饰酶基因型和BMD法结果一致。结论 VITEK 2C在检测鲍曼不动杆菌对阿米卡星的敏感性时有局限性,需要采用KB法替代检测。Objective To evaluate the performance of VITEK 2 COMPACT automatic bacterial identification system（ VITEK 2C） plus AST GN-13 for the susceptibility of Acinetobacter baumannii to amikacin. Methods Three methods [ broth microdilution（ BMD）, Kirby-Bauer（ KB）, and VITEK 2C] were used to determine the susceptibility of amikaein to 38 clinical isolates of Acinetobacter baumannii, and then the performances of VITEK 2C and KB were evaluated by using BMD as a reference method. Genes encoding aminoglycoside-modifying enzymes were amplified by multiple polymerase chain reaction （PCR）. Results Comparing BMD as the reference method, VITEK 2C plus AST-GN13 yielded major errors in 19 isolates （50.0%） and minor errors in 2 isolates （5.3%）. Multiple PCR results of aminoglyeoside-modifying enzyme gene were consistent with the results of BMD. Conclusions The VITEK 2C has limitations, which requires that KB should be used for the susceptibility of Acinetobacter baumannii to amikacin.

关 键 词：鲍曼不动杆菌 微量肉汤稀释法 氨基糖苷修饰酶 

分 类 号：R446.5[医药卫生—诊断学]