骨髓增生异常综合征患者SALL4和Nanog的表达及其临床意义  

Expressions of SALL4 and Nanog in patients with myelodysplastic syndrome and their clinical significance

作　　者：丁柳美[1] 黄红梅[1] 高松[2] 邬扬炯[2] 李莹[2] 化范例[2] 

机构地区：[1]复旦大学附属金山医院检验科,上海201508 [2]复旦大学附属金山医院血液科,上海201508

出　　处：《检验医学》2013年第8期681-684,共4页Laboratory Medicine

基　　金：上海市金山区科委科研项目(2010-3-10;2010-3-13)

摘　　要：目的探讨骨髓增生异常综合征(MDS)患者外周血及骨髓SALL4 mRNA和Nanog mRNA表达及其临床意义。方法运用逆转录聚合酶链反应(PCR)检测75例MDS患者外周血和骨髓及30名正常对照者外周血有核细胞SALL4 mRNA和Nanog mRNA表达,并比较各亚型MDS之间的表达差异。结果 MDS患者外周血及骨髓SALL4 mRNA表达均明显高于正常对照(χ2=22.92,P<0.01),骨髓标本表达明显强于外周血标本(Z=5.60,P<0.01);Nanog mRNA表达亦高于正常对照(F=88.78,P<0.01),但在骨髓中的表达与外周血相仿(t=0.898,P=0.371);SALL4 mRNA与Nanog mRNA表达在MDS各亚型之间差异有统计学意义(P<0.01),以RAEB-Ⅰ和RAEB-Ⅱ2个亚型患者表达最强。7例转化为急性髓性白血病的MDS患者外周血和骨髓SALL4mRNA和Nanog mRNA的表达均高于其他MDS患者(SALL4 mRNA外周血t=2.27,P<0.05;骨髓t=2.85,P<0.05。Nanog mRNA外周血t=5.20,P<0.01;骨髓t=5.51,P<0.01)。结论 MDS患者外周血和骨髓高表达SALL4 mRNA和Nanog mRNA,其表达高、低与MDS转归有一定的相关性。Objective To investigate the expressions of SALL4 mRNA and Nanog mRNA in peripheral blood and bone marrow in patients with myelodysplastic syndrome（MDS） and their clinical significance. Methods The reverse transcription polymerase chain reaction （PCR） was used to detect the expressions of SALL4 mRNA and Nanog mRNA in the peripheral blood and bone marrow of 75 patients with MDS and the peripheral blood of 30 healthy controls. The difference of the expressions between MDS subtypes was analyzed. Results The expression of SALL4 mRNA in peripheral blood and bone marrow of patients with MDS was significantly higher than that in healthy controls （X^2 = 22.92, P 〈 0.01 ）. The higher expression of SALL4 mRNA was found in bone marrow than peripheral blood（ Z = 5.60, P 〈 0.01 ）. The expression of Nanog mRNA was higher in patients with MDS than healthy controls（ F = 88.78, P 〈0.01 ）, but there was no difference between peripheral blood and bone marrow（ t = 0. 898, P = 0. 371 ）. There were statistical significance of SALL4 and Nanog mRNA among MDS subtypes （P 〈 0.01 ） , especially RAEB-Ⅰ and RAEB-Ⅱ. In 7 patients who evolved into acute myeloblastic leukemia, the expressions of SALL4 mRNA （peripheral blood t = 2.27, P 〈 0.05 ; bone marrow t = 2.85, P 〈 0.05 ） and Nanog mRNA （ peripheral blood t = 5.20, P 〈 0.01 ; bone marrow t = 5.51, P〈0.01） were significantly higher than the others. Conclusions SALL4. mRNA and Nanog mRNA are over- expressed in patients with MDS, and the expression correlates with the clinical outcomes.

关 键 词：SALL4 MRNA NANOG MRNA 骨髓增生异常综合征 

分 类 号：R446.11[医药卫生—诊断学]