原发性肝癌组织及细胞系中DNMT mRNA表达的检测及意义  

Determination and its significance of DNMT mRNA expression in Hep3B,HepG2 and hepatocellular cancer tissues

作　　者：高波[1] 李海平[1] 余宗涛[1] 吕军[1] 张吉才[1] 

机构地区：[1]湖北医药学院附属太和医院检验科,湖北十堰442000

出　　处：《检验医学》2013年第8期716-719,共4页Laboratory Medicine

基　　金：湖北省教育厅重大项目资助(Z20082401)

摘　　要：目的检测原发性肝癌组织及细胞系Hep3B、HepG2中甲基化转移酶(DNMT)mRNA的表达,分析乙型肝炎病毒(HBV)感染与DNMT mRNA表达之间的关系。方法应用实时荧光定量聚合酶链反应(PCR)检测44例原发性肝癌组织及2种肝癌细胞系中DNMT1、DNMT2、DNMT3A、DNMT3B、GAPDH基因mRNA表达水平。结果 32例血清HBV标志物阳性患者肝癌组织中DNMT1、DNMT3A和DNMT3B mRNA表达水平明显高于12例血清HBV标志物阴性患者肝癌组织(P<0.05)。DNMT1、DNMT2、DNMT3A、DNMT3B mRNA在HBV相关细胞系Hep3B中相对表达分别为1.26±0.15、0.76±0.15、1.26±0.20、0.66±0.13,在非HBV相关肝癌细胞系HepG2中的相对表达分别为0.64±0.11、0.62±0.12、0.48±0.12、0.36±0.10,Hep3B细胞系中DNMT1、DNMT3A、DNMT3B mRNA表达高于HepG2细胞系(P<0.01)。结论 HBV相关原发性肝癌组织和肝癌细胞系中存在DNMT mRNA表达增高;HBV感染可能刺激DNMT mRNA的表达,进而促进抑癌基因的甲基化。Objective To investigate the expression of DNA methyltransferase（DNMT） mRNA in Hep3B, HepG2 and hepatoeellular cancer tissues, and to analyze the relationship between hepatitis B virus （HBV） infection and DNMT mRNA expression. Methods Fluorescence quantitative polymerase chain reaction （PCR） was used to determine the mRNA expressions of DNMT1, DNM72, DNMT3A, DNMT3B mRNA and GAPDH genes in Hep3B, HepG2 and hepatocellular cancer tissues. Results In HBV-associated cancerous tissues,the mRNA expression levels of DNMT1, DNMT 3A and DNMT 3B mRNA were higher than those in non-HBV-associated cancerous tissues （ P 〈 0.05 ）. The expressions of DNMTI, DNMT2, DNMT3A and DNMT3B mRNA were 1.26 ± 0.15, 0.76 ± 0.15, 1.26 ± 0.20 and 0.66 ± 0.13 in Hep3B and 0.64 ± 0.11, 0.62 ± 0.12, 0.48 ± 0.12 and 0.36 ± 0.10 in HepG2. The expressions of DNMT1, DNMT3A and DNMI3B mRNA in Hep3B were higher than those in HepG2 （ P 〈 0.01 ）. Conclusions Overexpression of DNMT mRNA is detected in HBV-assoeiated Hep3B, HepG2 and hepatocellular cancer tissues. The HBV infection can stimulate the expression of DNMT mRNA, and involve the methylation of tumor-suppressor genes.

关 键 词：甲基化转移酶 乙型肝炎病毒 荧光定量聚合酶链反应 表达 

分 类 号：Q503[生物学—生物化学]