HPLC测定丹芍方提取物中丹皮酚、芍药苷的含量  

Quantitative Determination of Paeonol and Paeoniflorin in Extraction of Danshao Formula by HPLC

作　　者：代丽萍[1] 谢小龙[1] 鲁艳清[1] 王娟[1] 

机构地区：[1]河南中医学院,郑州450046

出　　处：《中国实验方剂学杂志》2013年第17期127-129,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省教育厅重点研究项目(12A360001);河南中医学院博士基金项目(BSJJ2010-02)

摘　　要：目的:建立HPLC同时测定丹芍方提取物中丹皮酚、芍药苷含量的方法。方法:采用ODSHYPERSIL色谱柱(4.6 mm×250 mm,5μm),乙腈-水梯度洗脱,柱温32℃,流速0.8 mL·min-1,检测波长230,274 nm。结果:丹皮酚、芍药苷分别在0～0.202 1,0～27.960 0μg线性关系良好,平均回收率分别为97.40%,101.35%,RSD分别为1.18%,0.48%。结论:3批样品测定结果表明,建立的方法简便、准确、重复性好,可以用于丹芍方提取物中丹皮酚、芍药苷的含量测定。Objective：To establish a method of HPLC for quantitative determination of paeonol and paeoniflorin in the extraction of Danshao Formula.Method：The separation was performed on the chromatographic column of ODS HYPERSIL（4.6 mm × 250 mm,5 μm）,and the mobile phase in the gradient elution program was acetonitrile-water.The column temperature was maintained at 32 ℃.The flow rate was 0.8 mL·min-1,and the detection wavelength was set at 274 nm and 230 nm respectively.Result：Paeonol and paeoniflorin showed good linear relationship in the range of 0-0.202 1,0-27.960 0 μg.The average recovery was 97.40%（RSD 1.18%）,101.35%（RSD 0.48%） respectively.Conclusion：The method is easy and accurate with higher repeatability,which can be used in the quality control of paeonol and paeoniflorin in the extraction of Danshao formula.

关 键 词：丹芍方 芍药苷 丹皮酚 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]