白及不同提取部位抗耐甲氧西林金黄色葡萄球菌的体内外活性  

Research on Antibacterial Active Fraction of Bletilla striata against Methicillin-Resistant Staphylococccus aureus

作　　者：彭芙[1,2] 万峰[2,3] 熊亮[2] 彭成[2] 陈放[1] 

机构地区：[1]四川大学生命科学学院,成都610225 [2]成都中医药大学中药资源系统研究与开发利用省部共建国家重点实验室,中药材标准化教育部重点实验室,成都610075 [3]四川省人民医院新津分院,成都611430

出　　处：《中国实验方剂学杂志》2013年第17期217-220,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十一五"科技支撑计划重点项目(2009BAI84B00)

摘　　要：目的:研究白及对"超级细菌"——耐甲氧西林金黄色葡萄球菌(MRSA)的抑菌作用及活性部位。方法:规范提取白及乙酸乙酯部位、正丁醇部位、萃取后水部位、醇提后水煎液部位等4个部位,采用琼脂平板二倍稀释法测定以上4个部位对金黄色葡萄球菌(MSSA)及MRSA共6株病原菌的MIC;用白及乙酸乙酯部位以两种给药方式对小鼠进行预防给药,测定白及对MRSA感染SPF小鼠的保护力。结果:乙酸乙酯部位和正丁醇部位对受试病原菌均具明显抑菌活性,4个部位中尤以乙酸乙酯部位的抑菌活性最强,其MIC为0.065～0.26 g·L-1;且白及乙酸乙酯部位对MRSA感染小鼠有很强的保护作用,其中尤以ip 0.5 g·kg-1的保护达到100%。结论:白及对MSSA和MRSA有明显的抑菌作用,乙酸乙酯部位是其主要的活性部位。Objective：To study antibacterial active fraction of Bletilla striata against methicillin-resistant Staphylococccus aureus（MRSA）.Method：Minimum inhibitory concentrations（MICs） of ethyl acetate extract,n-butanol extract,water extract after extraction and water decoction after extracting alcohol from B.striata against MRSA and S.aureus（MSSA） was assayed.Oral administration and intraperitoneal injection of ethyl acetate extract from B.striata were given to mice before they are infected by MRSA.Result：Ethyl acetate extract and nbutanol extract showed obvious antibacterial activity.Ethyl acetate extract had the highest activity with MIC 0.0650.26 g·L-1.Ethyl acetate extract could completely protect mice from MRSA through intraperitoneal injection with the concentration of 0.5 g·kg-1.Conclusion：Ethyl acetate extract is the main active fraction of B.striata against MRSA and MSSA.

关 键 词：白及 活性部位 体内外抑菌活性 耐甲氧西林金黄色葡萄球菌 

分 类 号：R285.5[医药卫生—中药学]