逍遥散对大鼠阿尔茨海默病模型前额叶皮质PP-2A和GSK-3β的影响  

Effect of Xiaoyao San on Prefrontal Cortex PP-2A and GSK-3 in Alzheimer's Disease Rat Model

作　　者：李高申[1] 刘建涛[2] 赵唯贤[2] 郭梅珍[1] 李宜培[2] 王保伟[2] 

机构地区：[1]黄河科技学院,郑州450006 [2]河南职工医学院,郑州451141

出　　处：《中国实验方剂学杂志》2013年第17期244-247,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省2012年度科技计划项目(122102310410);河南省教育厅青年骨干教师资助项目(2011GGJS-220)

摘　　要：目的:探讨逍遥散对阿尔茨海默病大鼠模型前额叶皮质相关指标的影响。方法:将清洁级4～5个月龄SD雄性大鼠随机分为4个组,模型组(B组)、西药组(C组)、中药组(D组)分别采用D-半乳糖腹腔注射和A-β1～42peptide双侧海马注射联合造模[用药剂量:D-半乳糖10 mL(100 mg·kg-1),每日1次,连续注射42 d;A-β每侧海马组织1次性注射2μL(10μg)],生理组(A组)仅用等体积生理盐水做相同的造模处理。造模完成后,C、D组每日分别用奥拉西坦溶液0.75 g·kg-1和逍遥散煎剂10.g-1ig干预,1次/d,连续28 d,用药体积均为5 mL·kg-1;A,B组采用等体积的生理盐水ig。ig结束后,4个组分别进行Y迷宫行为学检测,并对比分析。之后断头剥离前额叶皮质送检。结果:B,C,D两组与B组比较,其尝试次数和行为正确率均占明显优势,差异具有统计学意义(尝试次数P<0.05;行为正确率P<0.01);B组与A组比较,尝试次数和行为正确率降低,差异具有统计学意义(尝试次数P<0.05;行为正确率P<0.01);降低C、D组与A组比较差异无统计学意义。免疫组化染色显示,与B组比较,C组和D组PP-2A阳性细胞数量表达均明显增加,而GSK-3β阳性细胞数量表达均明显减少。结论:逍遥散能改善AD大鼠的空间记忆能力,与其能够上调AD大鼠前额叶的PP-2A的表达、抑制前额叶的GSK-3β的表达有关。Objective：To investigate effect of Xiaoyao San on related indexes of the prefrontal cortex in rats model of Alzheimer’s disease.Method：The male SD rats were randomly divided into 4 groups,model group（group B）,western medicine group（C group）,Chinese medicine group（D group） respectively by intraperitoneal injection of D-galactose and A-β1-42 peptide bilateral hippocampal injection molding（D-galactose 10 mL（100 mg·kg-1）,once a day,continuous injection for 42 d;A-β 2 μL（10 μg） once,normal group（group A） was given normal saline.After modeling,C and D groups were given oxiracetam solution and Xiaoyao San ig once for 28 days.Result：In C and D groups,compared with B group,the number of attempts and the correct behavior rate is obvious,the difference was statistically significant（P ＆lt; 0.05 and P ＆lt; 0.01）;In B group,compared with A group,number of attempts and the correct behavior rate is poor,the difference was statistically significant（P ＆lt; 0.05 and P ＆lt; 0.01）;there was no significant difference in C,and D groups compared with A groups.Immunohistochemical staining resultd showed that,compared with B group,C group and D group increased the expression of PP-2A positive cells significantly,while the number of GSK-3 positive cells were significantly reduced.Conclusion：Xiaoyao San can improve the spatial memory ability of AD rats,the expression is related to its up-regulatiing expression of PP-2A and inhibitory prefrontal GSK-3β.

关 键 词：逍遥散 阿尔茨海默病 糖原合成酶激酶-3Β 蛋白磷酸酶-2A 前额叶皮质 

分 类 号：R285.5[医药卫生—中药学]