藏药巴桑母酥油丸对放射线-化学复合损伤小鼠红系造血功能的影响及机制  

Effect of Traditional Tibetan Tonic Prescription Basangmu Suyou wan on Erythropoiesis Function and Underlying Mechanism in Mice with Joint Radiation and Chemical Injury

作　　者：降央泽仁[1] 杨林[2] 黄茜[1] 黄晓芹[1] 

机构地区：[1]成都中医药大学民族医药学院,成都611137 [2]四川省阿坝州人民医院,四川马尔康624000

出　　处：《中国实验方剂学杂志》2013年第17期275-278,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072893)

摘　　要：目的:探讨藏药巴桑母酥油丸对放射线--化学复合损伤小鼠红系造血功能的影响及可能机制。方法:采用外周血红细胞计数、造血祖细胞集落分析、实时荧光定量多聚酶链反应等方法,检测灌胃巴桑母酥油丸(0.75,1.5,3.0 g·kg-1)后放射线-化学复合损伤小鼠外周血红细胞(RBC)数及血红蛋白(Hb)量、骨髓红系造血祖细胞集落产率、肾脏红细胞生成素(Epo)和脾脏红细胞生成素受体(EpoR)mRNA表达量。结果:灌胃14,20 d后,巴桑母酥油丸中、高剂量组外周血RBC数显著高于空白组、模型组和低剂量组(P<0.05);灌胃20 d后,巴桑母酥油丸中剂量Hb含量显著高于空白组和模型组(P<0.05)。灌胃14 d后,巴桑母酥油丸中剂量组骨髓细胞的早期、晚期红系祖细胞(CFU-E,BFU-E)集落产率均显著高于空白组和模型组(P<0.01);中剂量组肾脏Epo mRNA相对表达量显著高于空白组和模型组(P<0.05)。结论:巴桑母酥油丸可以通过刺激肾脏Epo分泌、促进骨髓造血祖细胞增殖分化,进而促进放射线-化学复合损伤小鼠外周血RBC数及Hb含量的恢复。Objective：To explore the effect of traditional Tibetan medicine tonic prescription Basangmu Suyou wan on erythropoiesis and elucidate the underlying mechanism in mice with joint radiation and chemical injury.Method：Using a blood cell counter,a colony forming assay and fluorescent real-time quantitative PCR,the changes in peripheral erythrocyte count,hemoglobin content,erythroid hematopoietic progenitors colony forming proportion of bone marrow cell,kidney erythropoietin（Epo）,spleen Epo receptor（EpoR） mRNA were investigated after 0.75,1.5,3.0 g·kg-1Basangmu Suyou wan was gastrically given to the mice with joint radiation and chemical injury,and compared with those in the blank group as well as the control group given physical saline.Result：The erythrocyte count of blank,modle,low dose,middle dose,high dose group were（8.92 ±0.30）,（8.67 ±0.38）,（8.85 ±0.49）,（9.35 ±0.77）,（9.61 ±0.30） ×1012/ L respectively at 14 days.The counts of 14 days middle dose and high dose group were highen than those of the blank,the modle and of low dose group（P ＆lt; 0.05）.The hemoglobin contents were（157.00 ± 6.78）,（145.75 ± 37.81）,（162.09 ± 8.57）,（166.17 ± 9.40）,（162.00 ± 6.07） g·L-1 respectively at 20 days aften tneetment,the content of middle dose group was higher than those of the blank and modle group（P ＆lt; 0.05）.The bone marrow cell colong forming unit erythrocyte（CFU-E） and burst-forming unit erythroid（BFU-E） proportion in the blank,modle and the middle dose group were（56.56 ± 6.64）,（55.18 ± 7.07）,（64.31 ± 9.15） /5 × 104 bone marrow cell（BMC） and（31.68 ± 6.13）,（33.44 ± 6.5）,（40.69 ± 8.06） /5 × 104BMC respectively at 14 days,the proportion in the middle dose groups were more higher than those of the blank and modle groups（P ＆lt; 0.01）.The kidney Epo mRNA expression level of the blank,the modle and the middle dose group were（1.13 ± 0.53）,（0.92 ± 0.20）,（2.48 ±1.56） 2-ΔΔCtrespectively,the level

关 键 词：藏药 巴桑母酥油丸 放-化疗 红系造血 

分 类 号：R285.5[医药卫生—中药学]