雄黄肝脏毒性的病理形态学特征  

The Morphological Characteristics of Liver Toxicity by Realgar

作　　者：高双荣[1] 梁爱华[1] 戴宝强[1] 王丽芳[1] 李桂琴[1] 刘婷[1] 李春英[1] 曹春雨[1] 王海林[1] 易艳[1] 郝然[1] 赵雍[1] 回连强[1] 夏晶[2] 曹帅[2] 李丽敏[2] 季申[2] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]上海市食品药品检验所,上海201203

出　　处：《中国实验方剂学杂志》2013年第17期295-299,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81001692);国家"十一五"科技支撑计划项目(2006BAI55B02-03);国家科技重大专项(2009ZX09301005);中国中医科学院自主选题项目(ZZ03046)

摘　　要：目的:研究反复灌胃给予雄黄后,大鼠肝脏毒性的病理形态学特征,为安全、有效地使用雄黄提供科学依据。方法:随机将大鼠分为对照组和雄黄0.01,0.04,0.17 g·kg-1剂量组。各剂量组均每日灌胃给药1次,对照组给予高纯水,连续3个月。于给药后1,2,3个月和停药1,2个月后,测定血清生化指标,并在光镜下观察肝组织病理形态学变化。结果:连续灌服雄黄≥0.04 g·kg-13个月后,≥40%的肝细胞轻度浊肿、变性,炎细胞浸润,10%～20%动物出现片状肝细胞浊肿、嗜酸性变及灶状坏死等病变。停药1个月后,0.17 g·kg-1剂量组有50%的肝细胞可见轻度浊肿,间质少量炎症细胞浸润,其余各组与对照组比较均无统计学差异。给药2,3个月后,试验各组的活化部分凝因激酶时间(APTT)较对照组明显缩短。连续给药3个月及停药1个月后,凝血酶原时间(PT)也明显缩短。各组动物的其余生化指标与对照组比较未见明显差异。结论:大鼠连续灌服雄黄≥0.04 g·kg-13个月后,肝脏出现轻度的早期毒性病变;停药后,病变逐渐恢复至正常。Objective：To study the morphological characteristics of liver toxicity induced by repeated intra-gastric administration of Realgar in rats to provide a scientific evidence for safe and effective application of Realgar.Method：The rats were randomly divided into four groups：control group and Realgar 0.01,0.04,0.17 g·kg-1 groups.The rats were intra-gastrically treated with Realgar once a day for successively 90 days,while the control group was given ultra-filtrated water.Serum biochemistry indexes were measured,and pathological change of liver under microscope was observed at day 30,60 and 90.Result：In more than 40% of the liver cells in rats mild cloudy swelling,degeneration and inflammatory cell infiltration could be observed.10% to 20% of the animals appeared patchy hepatocyte swelling,eosinophilic degeneration,focal necrosis,etc.After repeated intra-gastric administration of Realgar,more than 0.04 g·kg-1 successively 90 days.50% of liver cells in 0.17 g·kg-1 group showed mild cloudy swelling and mild inflammatory cell infiltration in interstitial at day 30 after drug withdrawal.The other groups had no statistical difference compared with the control group.2 and 3 months after intra-gastric administration of Realgar,the APTT in the experimental groups was significantly shorter than the control group.Administration for 3 months and 1 month after withdrawal,the PT also significantly shorten.The biochemical indexes in each group had no significant difference compared with the control group.Conclusion：After repeated intra-gastric administration of Realgar more than 0.04 g·kg-1 successively 90 days,the early and mild liver toxicity was observed,which can gradually returned to normal after Realgar withdrawal.

关 键 词：雄黄 肝脏 毒性 病理 

分 类 号：R285.5[医药卫生—中药学]