通络方药对2型糖尿病大鼠心肌和主动脉iNOS基因表达的影响  

Effect of tongluo recipe on the expression ofiNOS mRNA in myocardium and aorta of type 2 diabetic rats

作　　者：李文桐 孙亮亮[2] 邹俊杰[2] 刘志民[2] 

机构地区：[1]南京军区上海警备区第一干休所,上海市200040 [2]第二军医大学附属长征医院内分泌科,上海市200003

出　　处：《老年医学与保健》2013年第4期221-223,共3页Geriatrics & Health Care

摘　　要：目的观察通络方药（Tongluo Recipe，TLR）对2型糖尿病大鼠心肌和主动脉诱导型一氧化氮合酶（iNOS）基因表达的影响，探讨TLR在2型糖尿病（type 2 diabetes，T2DM）的干预作用和应用价值。方法30只雄性健康清洁级SD大鼠随机分为3组：正常对照组、T2DM组、T2DM＋TLR干预组，每组10只。T2DM＋TLR干预组大鼠TLR按0．4g·kg^-1·d^1剂量灌胃，1次，d，持续给药12W后处死取心肌、主动脉组织待测。利用实时定量逆转录多聚酶链反应（RT-PCR）方法测定各组大鼠心肌、主动脉诱导型一氧化氮合酶iNOS的基因表达水平。结果与正常对照组比较，2型DM组大鼠心肌和主动脉iNOS的mRNA表达明显降低（P〈0．01、P〈0．05），而T2DM＋TLR干预组大鼠心肌和主动脉iNOS的mRNA表达较T2DM组明显升高（P〈0．01）。结论TLR可以增加T2DM大鼠心肌、主动脉iNOS的mRNA表达，从而增加NO合成，可能对T2DM具有防治意义。ObjectiveToinvestigatetheinterventionandapplicationvalueofTongluoRecipe （TLR） in type 2 diabetes by detecting the expression of iNOS mRNA in myocardium and aorta of type 2 diabetic rats. Methods Thirty SD healthy rats were randomly divided into 3 groups： normal control group, type 2 diabetes group, and type 2 diabetes ＋ TLR in-tervention group. There were 10 rats in every group. The rats in type 2 diabetes ＋ TLR intervention group were treated with TLR of 0.4 g·kg^-1 · d^1 dose via gavage. All rats were sacrificed after 12 weeks intervention and the myocardium and aorta of rats were obtained. Results Comparing with the normal control group, the levels of the expression of iNOS mRNA in myocardium and aorta in the type 2 diabetes group were significantly decreased （P〈0.01, P〈0.05）. But the levels of the expression of iNOS mRNA in the type 2 diabetes ＋ TLR intervention group were significantly increased as compared to the type 2 diabetes group （P〈0.01）. Conclusion TLR may treat and prevent T2DM via increasing the expression of iNOS mRNA in myocardium and aorta and further increasing NO synthesis.

关 键 词：通络方药 2型糖尿病 心血管疾病 一氧化氮合酶 基因 

分 类 号：R285.5[医药卫生—中药学]