重组人白细胞介素11对正常及骨髓抑制小鼠的促血小板生成作用  

Promotion of Thrombopoiesis by rhIL-11 in Normal and Myelosuppressed Mice

作　　者：左从林 徐明波 王勇波 易辉 冯宇霞 李涛 刘秀珍 黄向东 

机构地区：[1]北京双鹭药业有限责任公司,北京双鹭医药生物技术研究所,北京100850 [2]北京昭衍新药研究中心,北京100041

出　　处：《中国实验血液学杂志》2000年第1期24-30,共7页Journal of Experimental Hematology

摘　　要：本试验的目的是观察国产重组人白细胞介素 11(rhIL 11)皮下注射对正常小鼠和骨髓抑制小鼠的血液学效应。给正常小鼠rhIL 112 0 0及 4 0 0 μg/kg皮下注射 ,连续 7天 ,第 5天时外周血血小板数轻度升高 ,起效较平缓 ,停药后血小板数可在 4天内恢复到给药前水平。对骨髓抑制小鼠 ,rhIL 11的治疗可明显地减轻外周血小板计数的下降程度 ,促进血小板计数的恢复。rhIL 11在每天 10 0 - 4 0 0 μg/kg时对骨髓抑制小鼠均有良好疗效 ,治疗 13- 15天血小板计数恢复到照射前水平 ,在每天 4 0 0 μg/kg时起效稍快于 10 0及 2 0 0 μg/kg。上述 3个剂量所引起的血小板最大升幅十分接近。rhIL 11的升血小板作用与其促进骨髓巨核系祖细胞的增殖与成熟有关。上述结果表明 ,rhIL 11治疗无论对正常小鼠还是骨髓抑制小鼠均可提高外周血血小板数 ,提示rhIL 11可能是一种治疗血小板减少症的药物。Hematological effects of rhIL-11 on normal and myelosuppressed male BALB/c mice were observed. Mice were subcutaneously injected with rhIL-11 for 7 consecutive days, at the dose of 200 or 400?μg/kg per day, peripheral blood platelet counts were moderately elevated on 5 days after administration and returned to base level within 4 days after discontinuation of injection. In myelosuppressed mice, treatment with rhIL-11 significantly ameliorated the degree of thrombocytopenia, the recovery of thrombocytopenia was also significantly accelerated at the dose range of 100-400?μg/kg per day, and blood platelet counts reached pre-irradiated level after 13-15 days of treatment. The magnitudes of platelet count elevation were similar among groups of 100,200 and 400?μg/kg per day, although recovery appeared earlier in group of 400?μg/kg per day. Significant increases in CFU-Meg were observed both in normal and myelosuppressed mice. Our results suggest that rhIL-11 promotes the increase of peripheral blood platelets both in normal and myelosuppressed mice, and can be used as a potential therapeutic agent for thrombocytopenia induced by chemotherapy.

关 键 词：白细胞介素-11 血小板生成 血小板减少症 

分 类 号：R558.205[医药卫生—血液循环系统疾病] R979.5[医药卫生—内科学]