应用SCF+IL-2等细胞因子从脐血CD34^+细胞扩增T细胞  

Expension In Vitro of T Cells from Cord Blood CD34^+ Cells Stimulated with SCF and IL-2

作　　者：刘元林[1] 隋拥君[1] 张双喜[1] 郭子宽[1] 吴英[1] 毛宁[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《中国实验血液学杂志》2000年第1期48-51,共4页Journal of Experimental Hematology

摘　　要：肿瘤和艾滋病 (AIDS)的基因治疗或免疫治疗的研究需要大量的T细胞克隆。尽管T细胞来源于CD34+细胞 ,但由于需要胸腺组织的参与 ,使其在体外扩增大量细胞克隆非常困难。本研究用脐血单个核细胞作为辅助细胞 ,在SCF +IL 2等细胞因子的作用下 ,人脐血CD34+在此培养条件下 ,不断产生CD4 +或CD8+T细胞至少持续 3周。在培养扩增过程中 ,发现CD4 +和CD8+T细胞的比例有一个不断变化的动态过程 ,培养体系中可以检测到CD4和CD8双阳性细胞的存在 ;RT PCR可检测到负责TCR重排的RAG 2基因的表达 ,说明所获得的T细胞来源于CD34+细胞。本研究为从CD34+细胞获得大量的T细胞克隆提供了一个简便的体外培育体系。The generation of large quantities of novel human T cell clones ex vivo would make a wide range of gene-and immuno-therapies for tumor and AIDS possibly. Although it is well established that T cells are derived from CD34 + cells , the involvement of thymic fragments from either human or murine fetus makes the in vitro T cell perliferation very cumbersome. In this report, cord blood mononuclear cells were used as accessory cells to support the differentiation of CD34 + cells into naive T cells stimulated with SCF and IL-2. CD4 + and CD8 + T cells,under the cultural conditions, were continuously produced in vitro at least over a period of 3 weeks and their ratios changed gradually. CD4/CD8 double positive T cells and RAG-2 gene were existed, and RAG-2 gene, reponsible for TCR rearrangement, was expressed during the cell proliferation. Our study presents a simple culture system in vitro to acquire large quantities of naive T cell clones.

关 键 词：脐血 CD34^+细胞 T细胞 IL-2 干细胞因子 

分 类 号：R392.12[医药卫生—免疫学]