三氯乙烯对作业工人淋巴细胞DNA损伤的研究  

Research on DNA damage among workers exposed to trichoroethylene

作　　者：祖爱华[1] 李红梅[1] 王欣[1] 赖洪飘[1] 李晶[1] 崔晨[1] 莫民帅[1] 

机构地区：[1]广东省深圳市南山区疾病预防控制中心,518054

出　　处：《职业与健康》2013年第17期2113-2115,共3页Occupation and Health

基　　金：深圳市科技计划项目(医疗卫生类)(项目编号:201103295)

摘　　要：目的观察三氯乙烯作业工人的DNA损伤情况,探讨三氯乙烯对作业工人的遗传毒性。方法检测作业工人工作场所空气中三氯乙烯时间加权平均浓度,测定作业工人班后尿中三氯乙酸浓度,运用单细胞凝胶电泳(SCGE)检测50名三氯乙烯作业工人外周血淋巴细胞DNA损伤情况。结果三氯乙烯作业工人班后尿中三氯乙酸浓度大于对照组(P<0.01),DNA损伤情况与对照组比,差异有统计学意义(P<0.05),三氯乙烯作业工人尿中三氯乙酸浓度与淋巴细胞DNA损伤水平存在剂量-效应关系。结论长期接触三氯乙烯可导致作业工人淋巴细胞DNA损伤。[ Objective ] To observe the DNA damages and discuss the genotoxicity of trichloroethylene to the workers exposed to trichloroethylcne. [ Methods ] The time weighted average concentration of trichloroethylene in the air of working place were measured. The concentration of trichloroacetic acid in the urine of exposed workers after work was determined. The single cell gel electrophoresis （SCGE} was used to detect the DNA damage in peripheral blood lymphocytes of 50 workers. [ Results] The concentration of trichloroaeetie aeid in the urine of exposed group was higher than that of the control group （ P 〈 0.01 ） ; and the difference of DNA damage was also significant （ P 〈 0.05 ） ; A dose-effect relationship existed between the concentration of trichloroacetic acid in the urine of workers and the level of lymphocyte DNA damage. [ Conclusion] Long term exposure to trichloroacetie acid can lead to lymphocyte DNA damage of workers.

关 键 词：三氯乙烯 单细胞凝胶电泳 DNA损伤 

分 类 号：R135.1[医药卫生—劳动卫生]