活化脐血T细胞和NK细胞的抗原及细胞因子表达研究  

Comparative study on antigen expression of monocyte and cytokine levels of cord blood activated by interleukine-2 and interleukin-2+Anti-CD3 monoclonal antibody

作　　者：金雅磊[1] 王捷荣[1] 王红玲[1] 张渝侯[1] 

机构地区：[1]湖北医科大学附属第二医院,430071

出　　处：《中国民政医学杂志》2000年第6期335-338,共4页Medical Journal of Chinese Civil Administration

基　　金：湖北重点项目!(编号 96 2 -P110 4)基金资助

摘　　要：目的 :比较γIL - 2及抗 CD3m c Ab+γIL - 2对脐血单个核细胞 (MNC)的免疫表型及细胞因子的影响。方法 :采用间接玫瑰花结法实验测定 2 3例脐血 MNC表面的分化抗原 ,EL ISA双抗夹心法测定肿瘤坏死因子 -α(TNF-α)、白介素 - 6 (IL - 6 )、白介素 - 8(IL - 8)。结果 :经γIL - 2及抗 CD3mc Ab+γIL - 2激活的脐血 MNC其分化抗原发生明显变化。 1、两组与未活化相比 :CD3+ 、CD8+ 、CD16 + 、CD5 6 + 、CD19+ 、CD2 3+ 、HL A- DR↑ ,其表达 CD2 5亦均增加 ,但抗 CD3mc Ab+ γIL- 2激活组 >γIL- 2组 (P<0 .0 1) ,CD45 RA+均明显下降 ,但两组差异无显著性 (P>0 .0 5 ) ,CD34+无明显改变。 2、经抗 CD3m c Ab+ γIL- 2激活的 MNC,细胞集落增加 ,但 γIL- 2组无此作用。 3、细胞因子的改变 ,无论经 γIL- 2还是抗 CD3m c Ab+ γIL- 2激活的脐血 MNC上清液 ,其 IL- 6增高 ,但差异无显著性 (P>0 .0 5 ) ,TNF- α,IL- 8亦明显增高 ,但抗 CD3m c Ab组 >γIL- 2组 (P<0 .0 1)。结论 :脐血抗 CD3激活的杀伤细胞 (CD3AK)比 γIL- 2激活的L AK毒性作用更强 ,更有利于杀伤肿瘤细胞。Objective:To compare and analyse the antigen expression of monocyte and cytokine of human umbilical cord blood activated by γIL\|2 and γIL\|2+Anti\|CD3mcAb.Methods:By using indirect resette forming test,antigen expression rate of monocyte in 23 cases was detected.The levels of interleukin\|8(IL\|8)、interleukin\|6(IL\|6) and tumor necrosis factor α(TNF\|α) of 23 cases were measured by ELISA.Results:After activated by Anti\|CD3mcAb and/or γIL\|2,The antigen expression of monocyte and cytekine are obviously changed:(1) in γIL\|2 group and γIL\|2+Anti\|CD3mc Ab group,CD3\++、CD8 +、CD56 +、HLA DR\++\,CD19\++\,CD25\++↑,but γIL\|2+Anti\|CD3mcAb group>γIL\|2 group ( P <0 01),CD45RA\++↓,But there are no difference between two groups ( P >0 05).CD34\++ cells (also named hemopoietic stem cell) are no significant change.(2) The number of celluar colony is increased in Anti\|CD3mcAb+γIL\|2 group,but it is no change in γIL\|2 group.(3) The change of cytokine:Both of the levels of IL\|8、TNF α are increased in the sreum of cord blood in two groups,But γIL\|2+Anti\|CD3mcAb group>γIL\|2 group( P <0 01).The levels of IL\|6 in two groups is also higher than unactivated group.Conclusions:Anti\|CD3mcAb activated killer cells are stronger cytoxicity than lymphikine\|activated killer cells,therefore it maybe have advantage of killing tumor. [

关 键 词：白细胞介素 胎血 抗原CD3 T细胞 

分 类 号：R392.12[医药卫生—免疫学]