ghrelin在致渴条件下对大鼠饮水的影响  

THE EFFECTS OF GHRELIN ON WATER INTAKE UNDER DIPSOGENIC CONDITION

作　　者：李莹[1,2] 徐珞[1] 

机构地区：[1]青岛大学医学院病理生理学教研室,山东青岛266021 [2]菏泽医学专科学校

出　　处：《青岛大学医学院学报》2013年第5期390-392,396,共4页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家自然科学基金(No.30470642;No.30670780;No.31071014;No.81100260;No.81270460);山东省科技攻关项目(2008GG10002006);山东省卫生厅项目(2007HZ026);青岛市科技局项目(11-2-3-3-(2)-nsh)

摘　　要：目的探讨ghrelin在致渴条件下对水食摄入的调节。方法给予大鼠以下5种致渴刺激:①禁水36h;②用30g/L氯化钠代替饮用水;③外周注射高渗盐水;④侧脑室(ICV)注射血管紧缩素Ⅱ(AngⅡ);⑤外周注射高渗盐水+中枢注射AngⅡ。之后大鼠经ICV注射ghrelin0.4μg,观察大鼠摄食摄水量的改变。结果禁水36h后ghrelin可显著增加大鼠摄食量(t=4.900,P<0.05);饮用36h高渗盐水后ICV注射ghrelin大鼠摄食量显著增加(t=6.018,P<0.01);外周注射高渗盐水后ICV注射ghrelin大鼠摄食量显著增加(t=8.133,P<0.01),但摄水量显著减少(t=4.256～6.382,P<0.01);ICV注射AngⅡ大鼠摄食量无明显变化(P>0.05),但摄水量显著增加,ghrelin可显著降低由AngⅡ所致的饮水量增加(F=17.66～87.32,P<0.01);外周注射高渗盐水+中枢注射AngⅡ后,ghrelin可显著增加大鼠摄食量(t=6.348,P<0.05)。结论 ghrelin的作用并不仅限于摄食调节,也参与摄水调控。Objective To study the effects of ghrelin on water intake under dipsogenic condition. Methods The rats were exposed to one of the following five dipsogenic stimuli： ①water deprivation 36 h; ② drinking water replaced by 30 g/L NaCl; ③peripheral injection of hypertonic saline; ④ ICV injection of angiotensin Ⅱ fang Ⅱ） ; ⑤ peripheral injection of hyperton ic saline＋central Ang Ⅱ. The rats were then given ICV injection of ghrelin （0.4 t^g）, the changes of food and water intake were observed. Results After 36-hour water deprivation, ghrelin notably increased food intake （t =4.900,P〈0.05） ; After drinking hypertonic saline for 36 hours followed by ICV injection of ghrelin, food intake dramatically increased （t= 6.018,P〈0.01） ; After peripheral injection of hypertonic saline followed by ICV injection of ghrelin obviously increased food intake （t = 8.133, P〈0.01）, but water intake markedly decreased （t=4.256-6.382,P〈0.01）. ICV injection of AngⅡ did not change the food intake, but in- crease the waler intake, which could be lowered with ghrelin injection （F=17.66-87.32,P〈0.01）. After peripheral injection of hypertonic saline plus central injection of AngⅡ , ghrelin could significantly increase the food intake （t =6.348,P〈0.05）. Con- clusion Ghrelin involves not only in regulation of food intake, but also in water intake.

关 键 词：GHRELIN 饮水 摄食行为 血管紧张素Ⅱ 盐水 高渗 大鼠 

分 类 号：R338[医药卫生—人体生理学]