KK-42对日本沼虾蜕皮激素及其受体表达的影响  

Effect of KK-42 on Ecdysone and Ecdysteroid Receptor mRNA in Macrobrachium Nipponense

作　　者：刘方[1] 陈香丽[1] 郭爱莲[1] 宁黔冀[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2013年第4期124-127,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省高校科技创新人才支持计划(2009HASTIT022)

摘　　要：用1.95×10-4 mol.L-1的KK-42处理日本沼虾1min,不同时刻抽取血淋巴、解剖肝胰腺.HPLC测定血淋巴中20E滴度,RT-PCR测定EcR和RXR mRNA水平.结果:KK-42处理后3h,6h,20E滴度分别比对照组增加了121.71%(P<0.05)、268.96%(P<0.01);EcR mRNA水平在处理后6h,12h,分别比对照组提高了233.56%(P<0.01)、110.73%(P<0.05);RXR mRNA水平在处理后3h、12h,分别比对照组提高了195.53%(P<0.01)、80.83%(P<0.05).表明KK-42可显著提高血淋巴中20E的滴度,并诱导肝胰腺中EcR和RXR的基因转录.The spawns were soaked for 1 min in KK-42 solution at a concentration of 1.95 X 10-4 mol L-1 , and then hemolymph was extracted, and hepatopancreas were removed. Hemolymph 20 E titer was determined by HPLC. EcR and RXR mRNA level were assayed by RT PCR. The result showed that the titers of hemolymph 20 E significantly increased after KK- 42 treatment compared with the corresponding controls at 3 h（P〈0.05） and 6 h（P 〈0.01） ; the hepatopancreas F.cR mRNA level significantly increased compared with the corresponding controls at 6 h（P〈0.01） and 12 h（P〈0.05） after KK-42 treat- ment; the hepatopancreas RXR mRNA level significantly increased compared with the corresponding controls at 3 h（P〈0.01） and 12 h（P〈0.05）after KK-42 treatment. It indicates that KK-42 treatment enhanced ecdysone titer, and up-regulatecl the ex- pressions of EcR and RXR.

关 键 词：KK-42 日本沼虾 20E ECR RXR 

分 类 号：Q459[生物学—生理学]