磷酸化Akt和ERK在大鼠腹主动脉瘤中的表达  

Expressions of Akt and ERK Phosphorylation in Rat Model of Abdominal Aortic Aneurysm

作　　者：郑红波[1] 周洪莲[1] 武亚丽[1] 周丹[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院老年病科,武汉430030

出　　处：《天津医药》2013年第9期891-893,I0004,I0005,共5页Tianjin Medical Journal

基　　金：武汉市科技攻关计划资助项目(项目编号:200862123565)

摘　　要：目的观察磷酸化Ak(tp-Akt)和磷酸化ERK(p-ERK)在大鼠腹主动脉瘤模型中的表达情况,探讨腹主动脉瘤的发病机制。方法构建大鼠腹主动脉瘤模型并测量腹主动脉直径,计算直径扩张度;HE染色观察腹主动脉的病理改变;免疫组织化学技术和Western blot技术检测腹主动脉组织中p-Akt和p-ERK的相对表达量。结果腹主动脉瘤组主动脉扩张度(3.689±0.443)明显高于生理盐水组(1.175±0.159)和正常组(1),差异有统计学意义(P<0.05);HE染色显示,腹主动脉瘤组主动脉结构紊乱并有炎症细胞浸润;免疫组化结果和Western blot结果均显示p-Akt在腹主动脉瘤组中的表达明显高于生理盐水组和正常组(P<0.05);p-ERK表达差异不明显(P>0.05)。在腹主动脉瘤组中,p-Akt表达水平与主动脉扩张度呈正相关,p-ERK表达水平与主动脉扩张度无明显相关性。结论 PI3K/Akt信号通路可能参与腹主动脉瘤的发生与发展。Objective To observe the expressions of Akt and ERK phosphorylation in abdominal aortic aneurysm of rat model, and explore the pathogenesis of abdominal aortic aneurysm. Methods The rat model of abdominal aortic aneu- rysm was established. The diameter of abdominal aorta was measured and the extended rate of the aorta was calculated. HE staining was used to observe the change of pathology. Immunohistochemistry and Western blot methods were used to detect the expressions of Akt and ERK phosphorylation in the level of protein. Results The dilation of aorta was significantly high- er in abdominal aortic aneurysm group than that of saline group and normal group （P 〈 0.05）. HE staining showed structural disorder and inflammatory cell infiltration in abdominal aortic aneurysm group. The results of immunohistochemistry and Western blot results showed that phosphorylation of Akt expression was significantly higher in abdominal aortic aneurysm group than that of saline group and normal group （P 〈 0.05）. There was no significant difference in phosphorylation of ERK expression between three groups （P 〉 0.05）. Conclusion PI3K/Akt signaling pathway may be involved in the development of abdominal aortic aneurysm.

关 键 词：主动脉瘤 腹 磷酸化酶激酶 大鼠 SPRAGUE-DAWLEY 磷酸化AKT 磷酸化ERK 

分 类 号：R543.16[医药卫生—心血管疾病]