不同剂量白酒对大鼠内皮细胞微颗粒水平的影响  

Impacts of Different Doses of Alcohol on Rat Endothelial Microparticles

作　　者：张雅卓[1] 郑曼 蔡九英[1] 

机构地区：[1]辽宁医学院附属第一医院心血管内科,辽宁锦州121001 [2]东营市人民医院心内科

出　　处：《天津医药》2013年第9期904-905,共2页Tianjin Medical Journal

基　　金：辽宁省教育厅高等学校科研基金资助项目(项目编号:2009A446)

摘　　要：目的探讨不同剂量白酒对大鼠内皮细胞微颗粒水平的影响。方法 60只雄性SD大鼠随机分成4组:白酒大剂量组(A组)、中剂量组(B组)、小剂量组(C组)和对照组(D组),每组15只。白酒灌胃8周,采用流式细胞术检测各组大鼠血浆中CD31+/CD42-内皮微颗粒的水平,并比较其与白酒剂量的关系。结果与D组相比,A、B组大鼠血浆内皮细胞微颗粒水平显著升高(P<0.05),C组改变不明显(P>0.05)。结论大、中剂量白酒灌胃对大鼠血管内皮细胞功能有损害作用,呈明显的量效关系;小剂量白酒灌胃对大鼠血管内皮细胞功能无损害作用,是否有保护作用仍需进一步研究。Objective To discuss the impacts of different doses of alcohol on the level of microparticles （EMPs） of rat endothelial cells. Methods Sixty male SD rats were randomly allocated into four groups： high dose group （group A）, medium dose group （group B）, low dose group （group C） and the blank contrast group （group D）. There werel5 rats in each group. Rats were fed alcohol for 8 weeks. Flow cytometer was used to measure the level of circulating CD31＋/CD42- EMPs in four groups, and which was compared with the alcohol dosage. Results Compared with group D, the level of circulating EMPs was significantly increased in group A and B （P 〈 0.05）. There was no significant change in the level of circulating EMPs in group C （P 〉 0.05）. Conclusion The moderate and high doses of alcohol are harmful to the function of vascular endothelial cells in rats, which show a significant dose-effect relationship. The low dose of alcohol shows no effect on the function of vascular endothelial cells in rats. The protective effect of alcohol needs further investigation.

关 键 词：乙醇 内皮 血管 内皮细胞微颗粒 流式细胞术 大鼠 Sprague—Dawley 

分 类 号：R541.4[医药卫生—心血管疾病]