补肾益气方影响人早孕绒毛滋养细胞分泌功能的研究  

Effect of Kidney-replenishing Herb on the Secretion of Human Trophoblasts

作　　者：邢长英[1] 归绥琪[2] 陶敏芳[1] 滕银成[1] 

机构地区：[1]上海交通大学附属第六人民医院,上海200233 [2]复旦大学附属妇产科医院,上海200011

出　　处：《生殖与避孕》2013年第8期518-523,共6页Reproduction and Contraception

基　　金：上海市科委现代生物与制药基金(02D219115);国家自然科学基金(30572446)项目

摘　　要：目的：探讨补肾益气方调控人早孕母-胎界面内分泌免疫网络的机制。方法：收集正常孕6～8周行人工流产术后的绒毛，体外分离、培养人早孕绒毛滋养细胞。运用血清药理学方法，分别以体积分数10％和20％补肾益气方含药血清（分别为中药低剂量组和高剂量组）处理细胞24h、36h、48h，同时设立体积分数10％及20％对照组血清和空白对照组，ELISA法测定细胞培养上清液中白介素-10（IL-10）、干扰素-r（IFN—r）分泌水平，电化学发光免疫分析法测定孕酮（P）水平。结果：24h起，中药低、高剂量组IL-10水平高于血清对照组及空白对照组（P〈0．05）；并且48h时，中药高剂量组IL-10浓度高于低剂量组（P〈0．01）。IFN-r水平中药组与血清对照组相近（P〉0．05）。24h起，中药低剂量组和高剂量组的孕酮（P）水平均高于各对照组（P〈O．01），并且中药高剂量组高于中药低剂量组（P〈0．05）。结论：补肾益气方含药血清以剂量一时间依赖方式促进人早孕滋养细胞的IL-10、P分泌，可能是其调节内分泌免疫网络的作用机制之一。Objective： To explore the mechanism of kidney-replenishing herb regulating endocrine-immune network at the maternal-fetal interface during human first-trimester. Methods： The trophoblastas were isolated from human villus by trypsin digestion and cultured in vitro, then the trophoblasts were stimulated by 10% and 20% kidney-replenishing herb for 24 h, 36 h and 48 h, respectively. Each exposural group was matched to control group with same concentration serum. The concentrations of interleukin- 10 （IL- 10）, interferon-r （IFN-r） and progesterone （P） in the culture medium were detected by ELISA and electrochemiluminesce immunoassay. Results： The secretion level oflL-10 stimulated by 10% and 20% kidney-replenishing herb for 24-48 h increased significantly compared with those of serum control group （P〈0.05）, and the level of IL-10 stimulated by 20% kidney-replenishing herb for 48 h increased significantly compared with that of 10% kidney-replenishing herb （P〈0.01）. The IFN-）＇ level induced by kidney-replenishing herb was similar as that of serum control group （P〉0.05）. The secretion level of P treated with 10% and 20% kidney-replenishing herb for 24-48 h increased significantly compared with that of serum control group （P〈0.01）, and P level stimulated by 20% kidney-replenishing herb for 48 h increased significantly compared with that stimulated by 10% kidney-replenishing herb （P〈0.05）. Conclusion： Kidney-replenishing herb improves the secretion of IL-10 and P produced by trophoblasts in a time＇ and concentration-dependent manner, which may be the mechanism of kidney-replenishing herb regulating endocrine-immune network at the maternal-fetal interface.

关 键 词：补肾益气方 含药血清 滋养细胞 孕酮(P) 白介素 IO(IL-10) 干扰素-r(IFN-r) 

分 类 号：R285[医药卫生—中药学]