过表达S100A6对BMP9诱导的小鼠间充质干细胞C3H10T1/2成骨分化的影响  

Effect of Overexpressed S100A6 on Osteogenic Differetation of BMP9 Induced Mesenchynal Stem Cell C3H10T1/2

作　　者：叶立伟[1] 武睿[1] 段亮[1] 张昀源[1] 杨霞[1] 陈娴[1] 王海燕[1] 何通川[1] 周兰[1] 

机构地区：[1]重庆医科大学检验医学院临床检验诊断学教育部重点实验室,重庆400016

出　　处：《中国细胞生物学学报》2013年第8期1087-1093,共7页Chinese Journal of Cell Biology

摘　　要：以骨形态发生蛋白9(bone morphogenetic protein 9,BMP9)作为诱导小鼠间充质干细胞C3H10T1/2定向成骨分化的细胞因子,观察过表达S100A6对成骨分化的影响。用重组腺病毒AdBMP9与AdS100A6共感染C3H10T1/2细胞,随后检测成骨分化标志物,包括Runx2、碱性磷酸酶(alkaline phosphatase,ALP)、骨桥素(osteopontin,OPN)和钙盐沉积;检测方法包括免疫细胞化学、ALP染色、活性分析以及茜素红S染色;同时,用Western blot检测β-catenin的表达。过表达S100A6对所诱导的间充质干细胞C3H10T1/2成骨分化的早期指标Runx2的蛋白水平无明显影响(P>0.05;ICC法),对第7天的ALP和第18天钙盐的沉积也无明显影响(P>0.05);但是,能够引起第12天的OPN升高(P<0.05);同时,S100A6对BMP9诱导的C3H10T1/2细胞中的β-catenin水平的影响在第3、7天不明显(P>0.05),但在第12天时可致β-catenin水平下调(P<0.05)。过表达S100A6可促进BMP9诱导的C3H10T1/2细胞中OPN的表达,但对最终的成骨分化无明显影响。To investigate the effects of S 100A6 in BMP9-induced osteogenic differentiation of mesenchymal stem cell line C3H 10T 1/2, C3H10T1/2 cells were co-infected with the recombinant adenovirus AdBMP9 and AdS 100A6. Immunocytochemistry, alkaline phosphatase staining, activity analysis, and alizarin red S staining were used to detect the markers of osteogenic differetation, including Runx2, alkaline phosphatase （ALP）, osteopontin （OPN）, and calcium deposition; Western blot was used to measure the β-catenin expression. S 100A6 had nether significant effects on Rtmx2 protein levels at 3 d nor ALP at 7 d, and the same to calcium deposition at 18 d （P〉0.05）. But, it increased OPN at 12 d （P〈0.05）. Meanwhile, S100A6 decreased the β-catenin level at 12 d （P〈0.05）, but it didn＇t change the level of β-catenin at 3 d and 7 d （P〉0.05）. S100A6 over-expression can promote the OPN expression and decrease the β-catenin level in BMP9-induced C3H10T1/2 cells, but it has no significant effect on the final osteogenic differentiation.

关 键 词：S100A6 骨形态发生蛋白9(BMP9) C3H10T1 2 成骨分化 Β-CATENIN 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]