脂联素对糖尿病大鼠肝脏中PPARα、SREBP-1C、载脂蛋白A5表达及甘油三酯含量的影响  

Effect of adiponectin on the expression of PPARot, SREBP-1C, apoA5, and triglyceride in liver of diabetic rats

作　　者：阳琰[1] 高琳[1] 邓华聪[2] 晏永慧[1] 李显文[1] 王小英[1] 廖鑫[1] 张晗[1] 

机构地区：[1]遵义医学院附院内分泌科,563099 [2]重庆医科大学附一院内分泌科

出　　处：《中华内分泌代谢杂志》2013年第8期696-699,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：贵州省优秀科技教育人才省长专项资金项目，贵州省科技合作项目

摘　　要：目的观察脂联素对糖尿病大鼠模型肝脏中PPARα、胆固醇调节元件结合蛋白1C（SREBP-1C）、载脂蛋白A5（apoA5）表达及甘油三酯含量的影响。方法取60只雄性Wistar大鼠分成单纯糖尿病组（DM，n=20）、脂联素治疗组（Ad—APN＋DM，n=20）和对照组（CON，n=20）。每组大鼠各取10只，采用实时荧光定量逆转录-聚合酶链反应（RT—PCR）、Western印迹分别对大鼠的肝脏组织中PPARα、SREBP-1C及apoA5基因、蛋白表达水平进行检测，并检测肝脏组织中甘油三酯含量。结果（1）DM组肝脏中PPARα、apoA5基因及蛋白表达比对照组显著降低（P〈0．05），SREBP-1C基因、蛋白表达及甘油三酯含量比对照组显著增加（P〈0．05）；（2）脂联素干预组肝脏中PPARα、apoA5基因及蛋白表达较DM组增加（P〈0．05），而SREBP-1C表达下降（P〈0．05），甘油三酯含量明显降低（P〈0．05）；（3）脂联素干预组肝脏中PPARα、SREBP-1C、apoA5基因及蛋白表达较对照组差异无统计学意义（P〉0．05），甘油三酯含量比较也无显著性差异（P〉0．05）。结论脂联素可能通过上调肝脏中PPARα、apoA5表达，抑制SREBP-1C的表达，从而降低甘油三酯水平。Objective To observe the gene and protein expression of PPARα, sterol regulatory element binding proteins-lC （SREBP-1C）, and apolipoprotein （apo） A5 under adiponectiu intervention, as well as triglyeeride level in liver in diabetic rats. Methods Sixty male Wistar rats were assigned into 3 groups in random ： diabetic group（ DM, n = 20 ）, adiponectin treated group （ Ad-APN ＋ DM, n = 20 ）, and control group （ CON, n = 20 ）. 10 rats from each group were taken to detect the gene and protein expression of PPARc~, SREBP-1 C, and apoA5 in liver by real time-fluorescent quantitation reverse transcription-polymerase chain reaction and Western blot. The level of triglyceride in liver was determined. Results （ 1 ） The gene and protein expressions of PPARα, apoA5 were decreased in DM group（ vs CON, P〈0.05 ）. The expression of SREBP-1C and the level of triglyceride were increased （P〈0.05）. （2）The expression of PPARoc and apoA5 in Ad-APN ＋DM group were increased while the expression of SREBP-1C was decreased （ all P〈0.05 ）. （ 3 ） No difference was found in the expressions of PPARα, SREBP-1 C, and apoA5, as well as the levels of triglyceride between Ad-APN ＋ DM group and CON group （ P 〉 0.05 ）. Conclusions Adiponectin may upregulate PPARoL and apoA5 expressions but inhibit SREBP-1C expression in liver of diabetic rats, leading to decreased triglyceride content in liver of rats.

关 键 词：脂联素 糖尿病 实验性 PPARΑ 胆固醇调节元件结合蛋白1C 载脂蛋白A5 甘油三酯 

分 类 号：R587.1[医药卫生—内分泌]