Luminex技术与ELISA方法检测抗HLA抗体在肾移植急性排斥反应中的应用  

Comparison of Luminex vs. ELISA method to detect HLA antibodies in renal transplantation

作　　者：王庆华[1] 尚乐乐[1] 唐敏英[1] 王瑾[1] 吴琳[1] 谭建明[1] 

机构地区：[1]南京军区福州总医院全军器官移植研究所,福州350025

出　　处：《中华器官移植杂志》2013年第8期455-457,共3页Chinese Journal of Organ Transplantation

摘　　要：目的探讨用Luminex技术与酶联免疫吸附试验（ELISA）方法检测肾移植受者抗HLA抗体的结果差异及其与移植后早期急性排斥反应（AR）的关系。方法以2010年10月至2012年10月接受亲属活体肾移植的34例受者为研究对象，术前采集受者血清，同时应用Luminex技术与ELISA方法进行抗HLA-I类、Ⅱ类抗体检测，并且记录术后早期AR发生情况。结果用Luminex技术检测抗HLA-I类抗体阳性率为41．2％0（14／34），检测抗HLA-Ⅱ类抗体阳性率为38．2%（13／34）；用ELISA方法检测抗HLA-I类抗体阳性率为2．9％0（1／34），检测抗HLA-Ⅱ类抗体阳性率为8．8%,40（3／34）；两种方法检测抗HLA-I类、Ⅱ类抗体阳性率的差异有统计学意义（x2=14．46，P〈0．05；x2=8．17，P〈0．05）。用Luminex技术检测18例术前抗体阳性者中有8例术后早期发生AR（占44．4％）；16例术前抗体阴性者中，有2例术后早期发生AR（占12．5％）。用ELISA方法检测4例术前抗体阳性者中，有3例术后早期发生AR（占75．O％）；30例术前抗体阴性者中，有7例术后早期发生AR（占23．3O,／oo）。结论Luminex技术检测。肾移植受者抗HLA抗体的检出阳性率高于ELISA方法，对于移植后早期AR的监测可以提供有用的信息。Objective To compare Luminex vs. ELISA methods in detecting HLA antibodies in kidney transplant recipients and their relation to acute rejection. Method Blood samples from 34 kidney transplant recipients were collected and the HLA antibodies were detected by both Luminex and ELISA methods. The sensitivity and specificity of both methods for predicting the development of acute rejection were analyzed. Results Fourteen recipients （14/34, 41.17%） positive for HLA class I antibodies were detected by using Luminex method, whereas only I case （1/34, 2.9%） was detected with positive HLA class I antibodies by ELISA method （P〈0. 05）. Similarly, 13 recipients （13/34, 38. 24%） positive for HLA class 1I antibodies were detected by using Luminex method, whereas the positive rate of HLA class antibodies by using ELISA method was 8. 80%（3/34, P〈 0. 05）. The sensitivity and specificity of Luminex method for predicting the acute rejection were 80%and 92. 3%respectively, in comparison to 30% and 77. 4% respectively by ELISA method. Conclusion Compared to the traditional ELISA-based method, Luminex method has a better sensitivity and specificity for predicting the development of acute rejection.

关 键 词：酶联免疫吸附测定 微球体 荧光 移植物排斥 肾移植 

分 类 号：R699.2[医药卫生—泌尿科学]