肝功能正常的乙型肝炎病毒感染者肝组织共价闭合环状DNA的研究  

Quantitative analysis of intrahepatic covalently closed circular hepatitis B virus DNA in hepatitis B virusinfected patients with normal liver function

作　　者：江军[1] 陈仕祥[1] 徐文胜[2] 熊志远[1] 何维新[1] 周燕[1] 赵克开 陈玉[2] 缪晓辉[2] 范平[1] 

机构地区：[1]解放军第一一三医院感染科,宁波315040 [2]上海长征医院感染科,宁波315040 [3]解放军第四〇四医院感染科,宁波315040

出　　处：《中华传染病杂志》2013年第8期458-461,共4页Chinese Journal of Infectious Diseases

基　　金：十一五“艾滋病和病毒性肝炎等重大传染病防治”科技部重大专项课题（2008ZXl0002-006）

摘　　要：目的探讨肝功能正常的HBV感染者肝组织HBV共价闭合环状DNA（HBVcccDNA）与血清HBVDNA、肝组织总HBVDNA之间的相关性。方法实时荧光定量PCR检测125例肝功能正常的HBV感染者的血清、肝组织总HBVDNA和肝组织HBVcccDNA定量。正态分布数据采用直线相关分析，非正态分布数据采用非参数统计。结果HBV感染者血清HBVDNA为（5．87±1．47）lgIU／mL，肝组织总HBVDNA为（6．63±1．27）lg拷贝／l0-5细胞；HBVcccDNA为（5．66±0．95）lg拷贝／l0-6细胞。血清HBVDNA与肝组织总HBVDNA呈正相关（r=0．743，P〈0．01），血清HBVDNA与肝组织HBVcccDNA呈正相关（r=0．417，P〈0．01），肝组织总HBVDNA与肝组织HBVcccDNA呈正相关（r=0．443，P〈0．01）。肝组织HBVcccDNA在31～40岁年龄段最高，血清HBVDNA和肝组织总HBVDNA则在20～30岁年龄段最高。64例（51．2％）HBV感染者肝组织病理学提示为〈G2S2，HBVcccDNA水平与肝组织炎症活动度分级和纤维化分期无关（X2=0．048，P〉0．05）。结论肝功能正常的HBV感染者肝组织HBVcccDNA与肝内总HBVDNA、血清HBVDNA呈正相关，不同年龄HBV感染者血清和肝组织内HBV复制水平存在差异，HBVcccDNA水平与肝组织病理学无关。Objective To investigate the correlation between the intrahepatic hepatitis B virus （HBV） covalently closed circular DNA （cccDNA）, serum HBV DNA, and intrahepatie total HBV DNA in HBV-infected patients with normal liver function. Methods Liver biopsy specimens and serum samples were obtained from 125 asymptomatic HBV-infected patients with normal liver function. Serum HBV DNA, intrahepatic total HBV DNA and HBV cccDNA were quantitatively determined by real time fluorescence polymerase chain reaction （PCR）. Linear correlation analysis was used to determine the relationships between normally distributed data. For data with abnormal distributions, nonparametric analysis was used. Results In HBV-infected patients with normal liver function, serum HBV DNA level was （5.87±1.47） lg IU/mL,intrahepatic total HBV DNA level was（6.63±1.27） copy/10-6 cells and intrahepatic HBV cccDNA was （5.66±0.95） copy/10-6 cells. Serum HBV DNA was correlated positively with both the total intrahepatic HBV DNA （r=0. 743, P〈0.01） and with intrahepatic HBV cccDNA （r=0. 417, P%0. 01）. The total intrahepatic HBV DNA was correlated positively with intrahepatic HBV cccDNA （r = 0. 443, P 〈0. 01 ）. Intrahepatic HBV cccDNA was highest in patients aged from 31 to 40 years, while serum HBV DNA and intrahepatic total HBV DNA were highest in patients aged between 20 and 30 years. Sixty-four （51.2 M） patients were pathologically confirmed with histological inflammation activity 〈G2 and with fibrosis score 〈S2. HBV cccDNA was not correlated with liver inflammation and fibrosis （X2 =0. 048, P〉0.05）. Conclusions In HBV-infected patients with normal liver function, intrahepatic HBV cccDNA is positively correlated with both serum HBV DNA and intrahepatic total HBV DNA. The replication lev els of HBV in serum and in liver tissue are different among age groups. There is no correlation between intrahepatic HBV cccDNA and liver pathology.

关 键 词：肝炎 乙型 肝炎病毒 乙型 DNA 环状 DNA 病毒 

分 类 号：R512.62[医药卫生—内科学]