乙型肝炎病毒感染患者血清半乳糖凝集素-9含量及其临床意义  

Galectin-9 expressions in patients with hepatitis B virus infection and its clinical significance

作　　者：余姣[1] 万伟[1] 李云飞[2] 王丽艳[1] 尹伟[1] 范文瀚[1] 李成忠[1] 梁雪松[1] 万谟彬[1] 

机构地区：[1]上海长海医院感染科,200433 [2]上海中医药大学药物临床研究中心

出　　处：《中华传染病杂志》2013年第8期475-478,共4页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金资助项目（30972600）

摘　　要：目的探讨慢性乙型肝炎、乙型肝炎肝硬化、肝细胞癌和慢加急性肝功能衰竭患者血清中半乳糖凝集素-9（Galectin-9）的水平及其与临床生物化学指标间的相关性。方法选择慢性乙型肝炎20例，乙型肝炎肝硬化16例，肝细胞癌14例，慢加急性肝功能衰竭19例和健康对照11例。ELISA法检测血清中Galectin-9水平的同时检测ALT、AST、TBil、HBVDNA和国际标准化比值（INR）。统计学处理采用t检验和方差分析。结果Galectin-9在慢加急性肝功能衰竭组为（2．743±1．711）ng／mL、肝细胞癌组为（2．453±1．517）ng／mL、慢性乙型肝炎组为（1．460±1．331）ng／mL、乙型肝炎肝硬化组为（1．168±0．764）ng／mL、健康对照组为（0．330±0．139）ng／mL，与健康对照组比较，差异均有统计学意义（f值分别为-4．63、4．61、-2．86和-3．58，均P〈0．05）。患者血清中TBil水平与Galectin-9有相关性，且有统计学意义（R2=0．0268，P=0．007），而ALT、AST、HBVDNA、INR均与Galectin-9无显著相关性。结论Galectin-9在慢加急性肝功能衰竭患者中水平偏高，与TBil有显著相关性，从而对预测这类患者疾病进展和预后有一定的临床意义。Objective To understand serum Galectin-9 expressions in patients with chronic hepatitis B （CHB）, hepatitis B virus （HBV） related hepatic cirrhosis, hepatocellular carcinoma （HCC）, and HBV-related acute-on-chronic liver failure （HBV-ACLF）, and to explore its correlati6n with biochemical indexes of related diseases. Methods Twenty patients with CHB （male：13, female： 7）, 16 with HBV related hepatic cirrhosis （male：ll, female：5）, 14 with HCC （male：14, female：0）, 19 with HBV-ACLF （male： 15, female：4）, and 11 healthy controls （male：8, female：3） were enrolled. Serum Galectin-9 levels were detected by enzyme-linked immunosorbent assay （ELISA）. Levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, total bilirubin （TBil）, HBV DNA and international normalised ratio （INR） were also measured. Statistical analyses were performed by t test and analysis of variance. Results The levels of Galectin-9 were （2. 743±1. 711） ng/mL in HBV-ACLF group, （2. 453± 1. 517） ng/mL in HCC group, （1. 460± 1. 331） ng/mL in CHB group, （1. 168±0. 764） ng/mL in HBV related hepatic cirrhosis group, and （0. 330±0. 139） ng/mL in healthy controls. There were significant increased than those in healthy controls （t = -4.63, 4.61, -2.86, -3.58; all P〈0.05）. Serum TBil levels were significantly associated withGalectin-9 （R2 = 0. 0268, P=0. 007）, while the remaining indicators, such as ALT, AST, HBV DNA and INR, were not significantly correlated with the levels of Galectin-9. Conclusions Galectin-9 expression is significantly higher in patients with HBV-ACLF, and is correlated with TBil level, which can be regarded as an indicator of disease severity and prognosis of patients with HBV infection.

关 键 词：半乳糖凝集素类 肝炎 乙型 慢性 肝肿瘤 肝功能衰竭 肝硬化 酶联免疫吸附测定 

分 类 号：R512.62[医药卫生—内科学]