氯胺酮对未成年大鼠学习记忆与海马Egr-1表达的影响  

Effects of ketamine on learning and memory function and expression of Egr-1 in hippocampus of minor rats

作　　者：李晓丽[1] 朋立超[1] 葛建岭[1] 周国霞[1] 

机构地区：[1]复旦大学附属上海市第五人民医院麻醉科,上海200240

出　　处：《上海交通大学学报（医学版）》2013年第8期1060-1063,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市卫生局科研基金资助项目(20114218)~~

摘　　要：目的研究氯胺酮对未成年SD大鼠学习记忆能力和海马早期生长反应基因(Egr-1)表达的影响。方法未成年SD大鼠20只随机分为生理盐水对照组(Ns组)和氯胺酮组(Ket组)。Ket组给予氯胺酮50 mg/kg腹腔注射,Ns组则腹腔注射等容量的生理盐水1 mL,连续6 d。给药后24 h行Morris水迷宫实验,测定大鼠的学习记忆功能;于末次Morris水迷宫实验结束后1 h断头取脑,Real-Time PCR检测海马Egr-1 mRNA表达,Western blotting检测海马Egr-1蛋白的表达。结果与Ns组比较,Ket组大鼠隐蔽平台逃避潜伏期明显延长(P<0.05);活动总路径缩短,穿越平台次数减少(P<0.05)。Ket组大鼠海马Egr-1 mRNA和蛋白表达水平显著低于Ns组(P<0.05)。结论氯胺酮可导致未成年大鼠学习记忆能力降低,其机制可能与海马Egr-1表达下调有关。Objective To investigate the effects of ketamine on learning and memory function and expression of early growth responsive gene-1 （Egr-1） in hippocampus of minor rats. Methods Twenty minor SD rats were randomly divided into normal saline control group （Ns group） and ketamine group （Ket group）. Rats in Ket group were intraperitoneally injected with 50 mg/kg ketamine, and those in Ns group were administrated with same amount of normal saline for 6 d. Morris water maze test was adopted to explore the learning and memory function at 24 h after the final injection. The sections of hippocampus were obtained at 1 h of the final Morris water maze test, and the expression of Egr-1 mRNA and protein was determined by Real-Time PCR and Western blotting respectively. Results In Morris water maze test, the latency in place navigation in Ket group was significantly longer than that in Ns group （P 〈 0.05）, and the times of crossing the platform and total swimming distance in Ket group were significantly decreased compared with Ns group （P 〈 0.05）. It was revealed that the expression of Egr-1 mRNA and protein in Ket group was significantly lower than that in Ns group （P 〈 0.05）. Conclusion Application of ketamine in minor rats may result in learning and memory iInpairment, and the mechanism may be associated with the down-regulation of expression of Egr-1 in hippocampus.

关 键 词：氯胺酮 学习记忆 MORRIS水迷宫 早期生长反应基因 

分 类 号：R614[医药卫生—麻醉学]