线粒体COⅠ和16S rRNA片段确定近江蛏和缢蛏属的分类地位  

MOLECULAR IDENTIFICATION OF THE TAXONOMIC STATUS OF SINONOVACULA RIVULARIS AND GENUS SINONOVACULA USING MITOCHONDRIAL COⅠ AND 16S rRNA FRAGMENTS

作　　者：翁朝红[1,2,3] 谢仰杰[2] 肖志群[2] 任鹏[2] 王志勇[2] 桂建芳[1] 

机构地区：[1]中国科学院水生生物研究所,武汉430072 [2]集美大学水产学院,农业部东海海水健康养殖重点实验室,厦门361021 [3]中国科学院大学,北京100049

出　　处：《水生生物学报》2013年第4期684-690,共7页Acta Hydrobiologica Sinica

基　　金：福建省908专项(No.FJ908-02-01-08);浙江海洋学院“海洋渔业科学与技术”省重中之重学科开放课题(No.20100210)资助

摘　　要：对5种双壳类软体动物(近江蛏Sinonovacula rivularis、缢蛏Sinonovacula constricta、小刀蛏Cultellusattenuatus、尖刀蛏Cultellus scalprum和大竹蛏Solen grandis)的线粒体基因COⅠ和16S rRNA部分序列进行测序和分析,并结合GenBank中其他竹蛏超科和樱蛤超科物种COⅠ和16S rRNA片段,计算种间遗传距离,构建系统发育树,探讨近江蛏及缢蛏属的分类地位。结果表明,5个物种COⅠ和16S rRNA片段A+T含量均远高于G+C含量,近江蛏与缢蛏之间的碱基序列差异和遗传距离均已达到种间差异水平,确定近江蛏为缢蛏属的一个种。分别构建COⅠ(砂海螂为外群)和16S rRNA片段(密鳞牡蛎为外群)的Neighbor-Joining系统树,两者的拓扑结构都明确显示,缢蛏属为灯塔蛤科一个属,灯塔蛤科录属于竹蛏超科,而不录属于樱蛤超科。Partial sequences of mitochondrial gene COⅠ and 16S rRNA from five bivalve species（Sinonovacula rivularis,Sinonovacula constricta,Cultellus attenuatus,Cultellus scalprum and Solen grandis） were sequenced and analyzed.In the context of the sequences of other species of Solenoidae and Tellinoidea from GenBank,genetic distances were calculated.Phylogenetic trees（Neighbor-Joining） were constructed to determine the taxonomic position of Sinonovacula rivularis and genus Sinonovacula.The results showed that the A＋T content in COⅠ or 16S rRNA fragments was far more than that of G＋C.Sequence difference and genetic distance between S.rivularis and S.constricta in COⅠ and 16S rRNA reached a level of interspecies difference,and thus S.rivularis was confirmed to be a separated species of genus Sinonovacula.The topological structures of phylogenetic trees constructed on the basis of COⅠ and 16S rRNA fragments with Mya arenaria or Ostrea denselamellosa as the outgroup revealed that Sinonovacula,which was a genus of Pharella,should be incorporated into Solenoidae,but not Tellinoidea.

关 键 词：近江蛏 缢蛏属 COⅠ 16S RRNA 系统发育 

分 类 号：Q959.21[生物学—动物学]