程序性细胞死亡分子-1,白介素-22及C反应蛋白在银屑病患者血清及外周血单一核细胞中的表达  

Expression of programmed death-1,IL-22 and CRP in the peripheral blood mononuclear cells(PBMCs) of patients with psoriasis

作　　者：段继惠[1] 穆红[1] 唐志琴[1] 张国珺[1] 魏文国[2] 

机构地区：[1]天津市第一中心医院检验科,天津300192 [2]天津市第一中心医院皮肤科,天津300192

出　　处：《临床皮肤科杂志》2013年第9期522-524,共3页Journal of Clinical Dermatology

摘　　要：目的:研究协同刺激分子程序性死亡受体(PD)-1,白介素(IL)-22及C反应蛋白(CRP)与银屑病发病的相关性研究。方法:分别采用反转录实时荧光定量聚合酶链反应(FQ-RT-PCR)的方法检测52例银屑病患者及30例正常对照者外周血单一核细胞(PBMC)中PD-1的mRNA表达水平;采用流式细胞术(FCM)检测T淋巴细胞表面PD-1的表达百分率;采用酶联免疫吸附试验(ELISA)检测细胞培养上清液中IL-22的水平;采用全自动免疫分析仪检测血清中CRP的水平。结果:①银屑病组PBMC中PD-1的mRNA表达水平显著低于正常对照组(P<0.01);②活化后,T淋巴细胞表面PD-1表达百分率显著低于正常对照组(P<0.01);③活化后,银屑病组细胞培养上清液中IL-22含量显著高于正常对照组(P<0.01);④银屑病组血清CRP水平显著高于正常对照组(P<0.01)。结论:协同刺激分子PD-1对银屑病组患者体内T淋巴细胞活化增殖及细胞因子分泌的抑制作用减弱,可能参与了银屑病的发病机制。Objective： To investigate the expressions and significance of Programmed death-1 （PD-1）, IL-22 and CRP in PBMCs of patients with psoriasis. Methods： Sera and PBMCs were obtained from the venous blood samples from 52 patients with psoriasis vulgaris and 30 normal human controls. Reverse transcription- realtime quantitative polymerase chain reaction （FQ-RT-PCR） was used to examine PD-1 mRNA expression in peripheral blood monocytes. Flow cytometry （FCM） was used to detect PD-1 expression rate after activation. Automatic immune analysis machine was used to detect serum CRP level. The levels of IL-22 in culture supernatant of PBMCs were assayed by ELISA. The levels of CRP were assayed by automatic immune analysis machine. Results： （1）In the patients with psoriasis and controls, the relative expression level of PD-1 mRNA in PBMCs was 10.51±3.20 and 7.18±2.44 respectively （P〈0.01）. （2）After activation, the percentage of PD-1 on T cell was 5.06±3.31 and 7.99±4.09 respectively （P〈0.01）. （3）ELISA revealed that the level of IL-22 protein in the patients and controls was （124.02±24.44） ng/L and （58.29± 20.35） ng/L respectively （P〈0.01）. （4）Serum CRP was （4.53±2.49） mg/dL and （0.34±43.17） mg/dL respectively （P〈0.01）. Conclusion： The costimulating factor PD-1 plays a negative role on T cell responses, it maybe correlated to the pathogenesis of psoriasis.

关 键 词：银屑病 程序性死亡受体-1 白介素22 C反应蛋白 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]