丹参酮ⅡA通过诱导C/EBPβ表达促进人源NB4和MR2细胞系分化  

TanⅡA triggers the differentiation of NB4 and MR2 cells by up-regulated expression of C/EBPβ

作　　者：张开基[1] 王季石[1] 李健[2] 曹金竹[3] 羊裔明[2] 

机构地区：[1]贵阳医学院附属医院血液科,贵州贵阳550004 [2]四川大学华西医院血液科,四川成都610041 [3]贵阳护理职业学院护理系,贵州贵阳550081

出　　处：《基础医学与临床》2013年第9期1123-1128,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(30470748)

摘　　要：目的探索在丹参酮ⅡA(TanⅡA)诱导NB4和MR2细胞分化过程中C/EBPβ(CAAT/enhancer-bindingproteinβ)的表达。方法用TanⅡA干预NB4和MR2细胞120 h,通过形态学和膜表面标志检测NB4和MR2细胞的分化情况明确TanⅡA对NB4和MR2细胞的分化效应;0,0.1,1和10 mg/L TanⅡA干预NB4和MR2细胞,通过RT-PCR及Western blot测定C/EBPβRNA和蛋白水平表达的变化确定C/EBPβ与TanⅡA浓度的关系;通过膜表面标志检测NB4和MR2细胞的分化情况明确TanⅡA浓度与NB4和MR2细胞分化间的关系。结果 TanⅡA能诱导NB4和MR2细胞分化(P<0.05);TanⅡA能从RNA和蛋白水平诱导C/EBPβ表达升高并与浓度成正相关(P<0.05);TanⅡA能通过非剂量依赖的模式诱导NB4和MR2细胞分化,其引起最大分化效果的浓度为1 mg/L。结论 TanⅡA能有效促进NB4和MR2细胞分化;TanⅡA通过上调C/EBPβ的表达发挥分化效应;TanⅡA最强作用浓度为1 mg/L。Objective To Investgiate the expression of C/EBPβ in APL cells during differentiation induced by Tan II A. Methods Tan II A treated NB4 and MR2 cells 120h, the characterizations of differentiation were examined by morphology and membrane differentiation antigens ; The mRNA and protein expressions of C/EBPI3 in different concentrations of Tan II A-intervened APL cells were examined by RT-PCR and Western blot ; APL cells were treated with different concentrations of Tan II A, the characterizations of differentiation were examined by membrane differentiation antigens. Results Tan II A induced the differentiation of ATRA-sensitive and ATRA-resistant APL cells （ P 〈 0. 05 ） ; Tan II A dose-dependend upregulated the expression of C/EBPI3 in mRNA and protein （ P 〈 0. 05 ） Tan 11 A promoted the differentiation of the NB4 and MR2 cells in a dose-independent manner and exerted its stron- gest effect at a concentration of 1 mg/L. Conclusions Tan II A promotes the differentiation of ATRA-sensitive and ATRA-resistant APL cells and exertes its strongest effect at in Tan II A-induced differentiation.

关 键 词：丹参酮ⅡA C EBPβ 分化 

分 类 号：R285[医药卫生—中药学]