HO-1/CO系统抑制AngⅡ诱导的大鼠心肌细胞凋亡  

Heme oxygenase-1/carbon monoxide system inhibits angiotensin Ⅱ induced apoptosis of myocardial cells

作　　者：谷颖[1] 王爱红[1] 陈东昌[1] 鹿克风[1] 

机构地区：[1]山东大学附属省立医院心内科,山东济南250021

出　　处：《基础医学与临床》2013年第9期1150-1154,共5页Basic and Clinical Medicine

基　　金：山东省自然科学基金(y2007c080);山东省卫生厅科研基金(2007HZ067)

摘　　要：目的探讨血红素氧合酶-1/一氧化碳(HO-1/CO)系统对血管紧张素Ⅱ(AngⅡ)诱导的大鼠心肌细胞凋亡的影响。方法原代培养新生Wistar大鼠心肌细胞,随机分为:对照组、AngⅡ组、AngⅡ+氯化血红素(hemin)(HO-1诱导剂)组和AngⅡ+锌原卟啉-9(ZnppIX)(HO-1抑制剂)组。用real-time PCR及Western blot检测心肌细胞HO-1mRNA和蛋白的表达,比色法测定细胞培养上清液中碳氧血红蛋白(COHb)含量,流式细胞仪检测细胞凋亡。结果AngⅡ组心肌细胞HO-1 mRNA、蛋白、COHb含量和细胞凋亡均明显高于对照组(P<0.05),AngⅡ+hemin组HO-1mRNA、蛋白、COHb含量进一步升高(P<0.05),而细胞凋亡回降(P<0.05),但仍高于对照组(P<0.05),AngⅡ+ZnPPIX组仅细胞凋亡显著升高(P<0.05),其他指标无显著变化。结论 HO-1/CO系统对AngⅡ诱导的心肌细胞凋亡具有抑制作用。Objective To investigate the influence of heme oxygenase-1/carbon monoxide （HO-1/CO） system on apoptotic myocardial ceils induced by angiotensin II （Ang II ）. Methods Primary cultures of myocardial cells in neonatal Wistar rat were randomly divided cells into four groups. Real-time PCR and Western blot were used to analyz HO-1 mRNA and protein expression, colorimetric method was used for determination of carbon oxygen hemoglobin content in supernatant fluid medium, flow cytometry instrument was used to detect cells apoptosis. Results Ang II group of myocardial cell HO-1 mRNA, protein, COHb content and cell apoptosis were significantly higher than those in the control group （ P 〈 0.05 ）, Ang II ＋ hemin group HO-1 mRNA, protein and COHb content further raise （P 〈 0. 05 ）, while apoptosis rollback （P 〈 0. 05 ）, but still higher than those of the control group （ P 〈 0. 05 ）. In apoptosis Ang II ＋ ZnPPIX group significantly increased （ P 〈 0. 05 ）, other index didn＇t show significant change. Conclusions HO-1/CO system attenuates the myocardial cells apoptosis induced by Ang II.

关 键 词：血红素氧合酶-1 内源性一氧化碳 心肌细胞 凋亡 

分 类 号：R541.6[医药卫生—心血管疾病]