血红素加氧酶-1上调自发性高血压大鼠肝脏MMP-2与MMP-9表达  

Hemeoxygenase-1 up-regulates MMP-2 and MMP-9 expressions in liver of spontaneously hypertensive rats

作　　者：李炳蔚[1] 苑晓晨[1] 张晓艳[1] 王冰[1] 李宏伟[1] 刘淑英[1] 修瑞娟[1] 

机构地区：[1]中国医学科学院北京协和医学院微循环研究所卫生部微循环重点实验室,北京100005

出　　处：《基础医学与临床》2013年第9期1180-1185,共6页Basic and Clinical Medicine

基　　金：2011年中国医学科学院微循环研究所公益性科研院所基本科研业务费专项基金

摘　　要：目的探讨血红素加氧酶-1(HO-1)对自发性高血压大鼠(SHR)肝脏基质金属蛋白酶-2(MMP-2)与-9(MMP-9)表达的影响。方法大鼠随机分为4组:Wistar诱导组(WH)与SHR诱导组(SH)按15 mg/kg每天连续4天腹腔内注射浓度为8 g/L的氯化血红素(hemin)诱导HO-1表达;Wistar对照组(W)与SHR对照组(S)腹腔内注射等体积0.1 mol/L NaOH(pH 8.3)。检测大鼠尾动脉收缩压。免疫组化法分析肝脏HO-1及MMPs表达。明胶酶谱法检测血浆MMP-2及MMP-9的相对活性。结果 WH组与SH组肝脏HO-1、MMP-2与MMP-9表达量均显著高于W组与S组。HO-1诱导可显著降低SHR大鼠血压。S组血浆MMP-2及MMP-9相对活性显著高于W组。WH组与SH组血浆MMP-2及MMP-9相对活性显著高于W组与S组。结论 HO-1可上调SHR肝脏MMP-2与MMP-9表达。Objective To explore the effects of hemeoxygenase-1 （ HO-1 ） induction on matrix metalloproteinase 2 （MMP-2） and MMP-9 expression in liver of spontaneously hypertensive rats （SHR）. Methods Rats were randomly divided into 4 groups. Wistar induction group （WH） and SHR induction group （SH） ： 8 g/L hemin was ad- ministered to the animals intraperitoneally at a concentration of 15 mg/kg · d for 4 consecutive days to induce expression of HO-1 ; Wistar control group （W） and SHR control group （ S ） ： equal volume of 0. 1 mol/L NaOH （pH 8.3 ） was administered intraperitoneally as controls. Systolic blood pressure （BP） of rats was determined. The expressions of HO-1, MMP-2 and MMP-9 in liver were determined by immunohistochemistry. The relative activity of MMP-2 and MMP-9 in plasma were detected by gelatin zymography. Results The expression of liver HO-1, MMP-2 and MMP-9 was significantly higher in WH and SH group as compared with W and S group, respectively（P 〈 0. 01 ）. Induction of HO-1 significantly attenuated BP of SHR. The relative activity of MMP-2 and MMP-9 in rat plasma was significantly higher in S group as compared with W group （P 〈 0. 01 ）. The relative activity of MMP-2 and MMP-9 in rat plasma was significantly higher in WH and SH group compared with W and S group, respectively （ P 〈 0. 01 ）. Conclusions HO-1 up-regulates expressions of MMP-2 and MMP-9 in liver of SHR and the mechanisms need to be further elucidated.

关 键 词：血红素加氧酶-1 基质金属蛋白酶 高血压 

分 类 号：R544.1[医药卫生—心血管疾病]