重组人粒细胞巨噬细胞集落刺激因子对烧伤后残余创面转化生长因子β_1和血管内皮生长因子表达的影响  

Effects of recombinant human granulocyte-macrophage colony-stimulating factor on expression of TGF-β_1 and VEGF in residual burn wounds

作　　者：邱学文[1] 

机构地区：[1]南方医科大学南方医院烧伤科,广东广州510515

出　　处：《中国新药与临床杂志》2013年第8期637-640,共4页Chinese Journal of New Drugs and Clinical Remedies

基　　金：广东省医学科研基金项目(B2012225)

摘　　要：目的探讨局部应用重组人粒细胞巨噬细胞集落刺激因子(rhGM-CSF)对烧伤后残余创面转化生长因子β_1(TGF-β_1)、血管内皮生长因子(VEGF)表达的影响。方法 6例烧伤后残余创面患者,采用同体对照的研究方法,每例患者选取两处面积为16~25 cm^2烧伤后残余创面,随机分为试验组和对照组。试验组创面给予rhGM-CSF凝胶剂,对照组给予空白基质安慰剂,每日换药1次。于用药前及用药后7、14 d切取创面边缘组织标本,采用免疫组化、ELISA法检测TGF-β_1、VEGF的表达水平。结果用药前两组各项指标均无显著差异(P>0.05)。用药后7、14 d试验组TGF-β_1和VEGF积分光密度(ID值)分别为17 924±1 440、19 777±1 198和37 116±1 694、43 197±1 796,均高于对照组(15 653±1 258、17 199±1 780和21 706±1 339、23 322±1 555,P<0.01)。用药后7、14 d试验组TGF-β_1和VEGF含量分别为(2 588±218)、(2 734±202)pg·g^(-1)和(3 258±146)、(3 463±138)pg·g^(-1),均高于对照组[(2 429±136)、(2 562±100)pg·g^(-1)和(3 048±121)、(3 161±127)pg·g^(-1),P<0.05或P<0.01]。结论局部应用rhGM-CSF能促进创面TGF-β_1、VEGF的表达,有益于烧伤后残余创面的愈合。AIM To investigate the effects of recombinant human granulocyte- macrophage colony- stimulating factor （rhGM-CSF） on the expression of transforming growth factor-β1 （TGF-β1） and vascular endothelial growth factor （VEGF） expression in residual burn wounds. METHODS The study was a randomized and self-controlled clinical trial. Six patients with residual burn wounds were enrolled. Two residual burn wounds with area of 16 - 25 cm2 were collected from each patient and were randomly divided into group A （with treatment of rhGM-CSF gel） and group C （with treatment of placebo）. The wound dressing was changed daily. The skin tissue samples around the wounds before and at the 7 th and 14 th days after the treatment were harvested for evaluation of expression of TGF-β1 and VEGF by immunohistochemistry and ELISA. RESULTS No significant differences in the expression of TGF-β1 and VEGF were observed before the treatment （P 〉 0.05）. The values of integral optical density （ID） of TGF-β1 and VEGF examined by immunohisto-chemistry were significantly increased in the group A compared to those in the group C at both the 7 th and 14 th days after the treatment, respectively （TGF-β1, 17 924 ± 1 440 vs. 15 653 ±1 258 at the 7 th day and 19 777± 1 198 vs. 17 199± 1 780 at the 14thday; VEGF, 37 116± 1 694 vs. 21 706± 1 339 at the 7 th day and 43 197 ± 1 796 vs. 23 322 ± 1 555 at the 14 th day, all reached the statistic difference P 〈 0.01 ）. Similar up- regulation of TGF- β1 and VEGF expression in group A was observed by ELISA at the 7 th and 14 th days after the treatment （TGF-β1, （2 588± 218） pg·g^-1 vs. （2 429 ± 136） pg.g-i at the 7 th day, P 〈 0.05 and （2 734± 202） pg·g^-1 vs. （2 562 ± 100） pg·g^-1 at the 14 th day, P〈 0.05; VEGF, （3 258 ± 146） pg·g^-1X vs. （3 048 ± 121） pg.g-i at the 7 th day, P〈 0.01 and （3 463± 138） pg·g^-1 vs. （3 161 ± 127） pg·g^-1 at the 14 th day, P 〈 0.01）. CONCLUSION rhGM-CSF aceelerates the r

关 键 词：烧伤 粒细胞巨噬细胞集落刺激因子 伤口愈合 

分 类 号：R644[医药卫生—外科学]