激动素对D-半乳糖致衰雌性小鼠卵巢和子宫的影响  

Effect of kinetin on ovary and uterus in D-galactose-induced female mouse model of aging

作　　者：孙江宏[1] 刘玉梅[2] 曹统[1] 欧阳五庆[1] 

机构地区：[1]西北农林科技大学动物医学院,杨凌712100 [2]河南科技大学动物科技学院,洛阳471003

出　　处：《生理学报》2013年第4期389-394,共6页Acta Physiologica Sinica

基　　金：supported by the National Natural Science Foundation of China(No.31101779)

摘　　要：本文旨在研究激动素对氧化损伤雌性小鼠卵巢和子宫的影响。采用普通级雌性昆明小鼠80只,以D-半乳糖复制小鼠衰老模型。按体重随机分为正常组、D-半乳糖模型组、25mg/kg激动素组和50mg/kg激动素组。通过检测小鼠的发情周期、卵巢和子宫湿重及脏器指数、雌二醇(E2)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)和总蛋白含量以及卵巢形成成熟卵母细胞的能力,观察激动素对D-半乳糖致衰雌鼠卵巢和子宫的作用。结果显示,与D-半乳糖模型组相比,25mg/kg和50mg/kg激动素组小鼠发情周期变短,卵巢和子宫湿重显著增加,血清E2含量显著提高;同时,卵巢和子宫的SOD及GSH-Px活性和总蛋白含量均显著增高,而MDA的含量则显著降低;而卵巢形成成熟卵母细胞的数量也极显著增加。以上结果表明,激动素能抵抗卵巢和子宫的氧化损伤,预防因卵巢氧化损伤而造成的雌激素分泌量低下、成熟卵母细胞减少问题,改善小鼠的发情周期,维持卵巢和子宫的器官功能并保持其活力。The present study was to investigate the effect of kinetin on ovary and uterus of D-galactose-induced female mouse model of aging. Aging female mice model caused by D-galactose were used as model group, the aging model mice intragastrically admin- istered with kinetin solution （daily 25 mg/kg or 50 mg/kg） were used as kinetin groups, and the mice with solvent as normal group （n = 20）. To detect the effects of kinetin, estrous cycle, estradiol content, ovarian and uterine wet weight and organ index, SOD and GSH-Px activities, MDA and total protein contents, as well as the reserve function of ovaries were examined. The results showed that, kinetin-induced changes in two kinetin groups were observed, compared with the model group： （1） the estrous cycle was short- ened; （2） serum estradiol content was significantly increased; （3） the wet weights of the ovary and uterus were increased significantly; （4） SOD and GSH-Px activities of ovary and uterus were significantly higher; （5） the MDA contents of the ovary and uterus were re- duced significantly; （6） total protein contents of the ovary and uterus were increased significantly; （7） the numbers of mature oocytes in fallopian tubes were increased significantly. The results show that kinetin can protect ovary and uterus against oxidative damage, prevent low estrogen secretion caused by ovarian oxidative damage, shorten the estrous cycle in mice, and eventually maintain ovari- an and uterine vitalities.

关 键 词：激动素 卵巢 子宫 抗氧化 抗衰老 

分 类 号：S852.23[农业科学—基础兽医学]