α-甘露聚糖肽作为甲型流感疫苗佐剂的免疫效果  

Immunologic effects of mannatide as a new adjuvant of H1N1 split vaccine

作　　者：冯素玲[1] 张卉[2] 任淑婷[3] 赵兴华[1] 廖怡然[1] 王冰[3] 

机构地区：[1]西安交通大学医学院药理学系,陕西西安710061 [2]西安交通大学医学院药学系,陕西西安710061 [3]西安交通大学医学院病理系,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2013年第5期604-608,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金："863"重大科技专项资助项目(No.2010AA022907;2012AA02A406)~~

摘　　要：目的研究α-甘露聚糖肽作为甲型流感疫苗佐剂的免疫效果。探索α-甘露聚糖肽作为鼻黏膜免疫佐剂的可行性。方法分别通过肌注或滴鼻方式给Balb/c小鼠接种α-甘露聚糖肽甲型流感疫苗;采用ELISA法检测血清中特异性IgG和鼻、肺灌洗液中sIgA抗体水平;采用MTT法进行淋巴细胞增殖试验。结果在肌注接种和滴鼻接种的量效实验中,佐剂疫苗组的血清IgG水平及鼻、肺灌洗液sIgA水平可达到单纯疫苗组2倍左右;佐剂疫苗组脾淋巴细胞体外刺激增殖率亦高于单纯疫苗组,且有显著性差异;在肌注接种的时效实验中,佐剂疫苗组血清IgG和鼻、肺灌洗液sIgA水平较单纯疫苗组在12周内一直保持在较高的水平。结论在肌注接种时,α-甘露聚糖肽能够同时促进体液免疫和细胞免疫应答,具有佐剂效应;在鼻黏膜接种时,加入α-甘露聚糖肽也可以增加甲型流感疫苗的免疫效果,因而α-甘露聚糖肽具有作为黏膜免疫佐剂的潜力。Objective To study the immunologic effects of mannatide as the adjuvant of H1N1 split vaccine and explore mannatide’s potential as a mucosal immune adjuvant. Methods H1N1 split vaccine was inoculated intramuscularly or intranasally to Balb/c mice in combination of mannatide. ELISA assay was employed to test the serum specific IgG level and the sIgA antibody level in nasal and bronchoalveolar lavages. MTT assay was used to perform the lymphocyte proliferation test. Results In the dose-effect test, the serum IgG antibodies and the sIgA levels in the nasal and bronchoalveolar lavages of mannatide adjuvant H1N1 vaccine groups were significantly higher than those of H1N1 vaccine group, either intramuscularly or intranasally. Stimulation of T lymphocytes after adjuvant immunization was stronger; the proliferation was significantly enhanced after stimulation with ConA in vitro. In the time-effect tests, the IgG and sIgA levels of adjuvant vaccine group remained higher than those of vaccine group for 12 weeks. Conclusion The results of our research suggest that mannatide put in intramuscular inoculation is a potential adjuvant which can promote both humoral and cellular immune responses. Mannatide put in intranasal inoculation can enhance the immunologic effect of H1N1 split vaccine. Therefore, mannatide has the potential of being a mucosal immune adjuvant.

关 键 词：α-甘露聚糖肽 免疫佐剂 甲型流感疫苗 黏膜免疫 SIGA IGG 

分 类 号：R967[医药卫生—药理学]