黄芩煎剂对肝硬化内毒素血症预防性实验的量效研究  

The dose-effect study on preventive experiment of scutellaria decoction on liver cirrhosis endotoxemia

作　　者：刘昳[1] 邱根全[1] 何锦妮[1] 叶峰[2] 杨雪亮[2] 

机构地区：[1]西安交通大学医学院第一附属医院中医科,陕西西安710061 [2]西安交通大学医学院第一附属医院传染科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2013年第5期674-677,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科技攻关项目(No.2009-j31)~~

摘　　要：目的研究黄芩煎剂对肝硬化内毒素血症形成的预防作用,并探讨其量效关系。方法四氯化碳联合胆固醇、乙醇建立肝硬化大鼠模型,将鼠模型分为黄芩煎剂大剂量组(12g/kg)、中剂量组(6g/kg)、小剂量组(3g/kg)、正常对照组、模型对照组及诺氟沙星组。实验过程中观察大鼠的一般情况、体重;实验结束时处死动物,检测血清内毒素水平、肝功能变化以及肝脏的纤维化程度。结果治疗结束时,黄芩煎剂大、中、小剂量组及诺氟沙星组大鼠的肝组织纤维化程度显著低于模型组(P<0.05),大鼠体重较模型组显著增加(P<0.05),内毒素血症水平较模型组显著降低(P<0.05),ALT水平上升显著低于模型组(P<0.05),白蛋白(AL)水平明显高于模型组(P<0.05);其中大剂量组的预防效果最好,中、小剂量组之间无显著差别,诺氟沙星组疗效最差。结论黄芩煎剂能够预防大鼠肝硬化内毒素血症的形成,且具有一定的量效关系。Objective To study the preventive effect of scutellaria decoction on cirrhosis endotoxemia and to explore the dose-effect relationship. Methods Carbon tetrachloride combined with cholesterol and ethanol was used to establish rat model of liver cirrhosis, and rats were divided into scutellaria decoction high-dose （12g/kg）, medium-dose （6g/kg） and low-dose （3g/kg） groups, normal control group, model control group and norfloxacin group. During the experiment, the rats’ general condition and body weight were observed; at the end of the experiment, the rats were executed to detect serum endotoxin level, liver function changes and liver fibrosis degree. Results At the end of treatment, liver fibrosis degree of rats in high-dose, medium-dose and low-dose treatment groups and norfloxacin group was significantly lower than that in model group （P〈0.05）; the rats’ body weight increased significantly compared with that in model group （P〈0.05）; endotoxemia level was significantly lower than that in model group （P〈0.05）. ALT level increase was significantly smaller than that in model group （P〈0.05）; albumin level was significantly higher than that in model group （P〈0.05）. The high-dose group had the best preventive effect, medium-dose and low-dose groups did not significantly differ, and norfloxacin group had the poorest efficacy. Conclusion Scutellaria decoction can prevent the formation of endotoxemia in rat liver cirrhosis, and they have a certain dose-effect relationship.

关 键 词：黄芩 肝硬化 内毒素血症 预防 量效关系 

分 类 号：R259[医药卫生—中西医结合]