凡纳滨对虾信号转导及转录激活因子(Lv-STAT)基因的克隆及表达特征分析  

Molecular cloning and expression pattern analysis of signal transducer and activator of transcription(Lv-STAT)from the Litopenaeus vannamei

作　　者：张铃[1] 刘逸尘[1] 张亦陈[1] 孙妍 耿绪云 孙金生[1,2] 

机构地区：[1]天津师范大学生命科学学院,天津市动植物抗性重点实验室,天津300387 [2]天津市水生动物疫病预防控制中心,天津300221

出　　处：《水产学报》2013年第8期1140-1146,共7页Journal of Fisheries of China

基　　金：国家“九七三”项目(2012CB114405);国家“八六三”高技术研究发展计划(2012AA092205,2012AA10A401);国家科技支撑计划项目(2011BAD13B04,2011BAD13B07);天津市自然科学基金项目(10JCYBJC09200);天津师范大学博士基金项目(52LX18,52LX19)

摘　　要：信号传导及转录激活因子(STAT)是生物体内重要信号通路JAK/STAT的关键组分,在机体免疫、生长、发育等方面起到重要作用。实验从凡纳滨对虾血细胞中克隆获得了STAT基因的全长cDNA序列(Lv-STAT,GenBank accession number:KC779541),其ORF区全长2 373 bp,编码790个氨基酸,预测的分子量为90.68 ku,等电点为5.91。利用SMART在线软件分析表明,该基因编码的蛋白主要结构域由STAT_int、STAT-α、STAT-β和SH2 domain 4部分组成,但不具有信号肽结构。将Lv-STAT与来源于其它物种的STAT进行氨基酸多序列比对后发现,该基因家族中的关键位点在Lv-STAT中同样保守。系统发生分析结果显示,Lv-STAT与斑节对虾、中国明对虾的STAT亲缘关系最近。在组织表达模式分析基础上进一步研究了该基因应答WSSV感染的表达变化特征,发现在感染后早期(0.5、1和3 h),该基因在血细胞中均呈显著上调表达趋势,上述研究表明该基因在一定程度上参与了凡纳滨对虾体内由WSSV引发的先天免疫应答过程。Signal transducer and activator of transcription factor（STAT） is an important part of JAK/STAT signaling pathway whose function performs in immunity, growth and development. In this research, the full length cDNA of STAT gene（Lv-STAT） was cloned from hemocytes of Litopenaeus vannamei. Lv-STAT has a 2 373 bp ORF encoding 790 aa with a predicted molecular weight of 90.68 ku and calculated isoelectric point of 5. 91. The SMART online software analysis showed that functional domains of Lv-STAT were composed of four components,STAT_int, STAT- α, STAT- β and SH2 domain. It didn＇ t have any signal peptide. The key sites of STAT gene family in Lv-STAT are highly conserved. Phylogenetic analysis shows that Lv-STAT is most closely related to P. monodon STAT, F. chinensis STAT and human STATsA and STATsB. The results of gene expression show that Lv-STAT was mainly expressed in hemocytes and hepatopancreas. The expression profiles of Lv-STAT in hemocytes and hepatopancreas were changed significantly （ P 〈 0.01 ） in the early period after WSSV （ White Spot Syndrome Virus） challenging （ 3 hpi）. The result suggests that Lv-STAT might take part in the antiviral innate immune response of shrimp triggered by WSSV.

关 键 词：凡纳滨对虾 STAT 表达模式分析 先天免疫 

分 类 号：Q785[生物学—分子生物学] S917.4[农业科学—水产科学]