HPLC法同时检测皮革和毛皮中游离甲醛、戊二醛  

Determination of Free Formaldehyde and Glutaraldehyde by HPLC in Leather and Fur

作　　者：钱晓晓[1] 陈卫琴[1] 唐旭东[1] 

机构地区：[1]温州市质量技术监督检测院,浙江温州325100

出　　处：《中国皮革》2013年第17期17-19,27,共4页China Leather

摘　　要：建立一种快速检测皮革和毛皮中游离甲醛、戊二醛的高效液相色谱法。以C18（150mm×4．6mm×5μμm）色谱柱为分离柱，以乙腈、水为流动相，流速1．0mL／min，二极管阵列检测器，检测波长360nm，外标法定量。结果表明：在0．1—2．0μg/mL范围内，甲醛、戊二醛线性关系良好，回收率在87％～102％之间。方法快捷准确、重现性好，精密度和检出限均可满足检测工作需要，适合皮革和毛皮中游离甲醛、戊二醛的同时检测。A rapid method for simultaneous determination of free formaldehyde and glutaraldehyde by HPLC in leather and fur was described. The HPLC conditions were C18 （ 150mm × 4.6mm × 5 μm） column with acetonitrile - water as mobile phase and the flow rate was 1.0mL/min. The photodiode array detector wavelength was at 360nm. The results show that the linear range for formaldehyde and glutaraldehyde is between 0.1 -2.0μg/mL,and the average recovery rate is at the ragne of 87%- 102%. The method is fast and accurate with good reproducibility and precision. The detection limits could meet the need for testing free form-aldehyde and glutaraldehyde in leather and fur.

关 键 词：游离甲醛 戊二醛 同时检测 皮革和毛皮 HPLC 

分 类 号：TS57[轻工技术与工程—皮革化学与工程]