葛根素对大鼠离体肠道平滑肌运动的影响及其机制  

The effect and mechanism of puerarin on the activity of isolated intestinal smooth muscle of rats

作　　者：刁汇玲[1] 孙晖[1] 高金祥[2] 马中女[1] 田伟[1] 马春蕾[1] 

机构地区：[1]滨州医学院生理教研室,山东滨州256400 [2]滨州医学院附属医院

出　　处：《中国老年学杂志》2013年第16期3897-3899,共3页Chinese Journal of Gerontology

基　　金：山东省自然科学基金(No.ZR2010HM011);山东省高等学校科技计划(No.J13LL71);滨州医学院科技计划(No.BY2007KJ02)

摘　　要：目的探讨葛根素对大鼠离体肠道平滑肌收缩活动的影响及其作用机制。方法应用PowerLab信号处理仪观察葛根素对大鼠离体肠道平滑肌收缩活动的影响及诱导型NO合酶(iNOS)是否介导葛根素对肠道平滑肌的作用;应用NO试剂盒测定葛根素对结肠内NO含量的影响;应用Western印迹观察葛根素是否上调结肠中iNOS的含量。结果 0.12、0.24、0.48 g/L葛根素对各段肠道平滑肌运动都有明显抑制作用,并随浓度增加而加强。应用SMT(iNOS抑制剂)对结肠平滑肌预处理后再加入葛根素,其对平滑肌作用减弱。0.12、0.24、0.48 g/L葛根素都可提高结肠平滑肌内NO和iNOS含量。结论葛根素可抑制肠道平滑肌的收缩活动,其机制可能与肠内iNOS表达上调有关。Objective To investigate the effects of puerarin on the activity of isolated intestinal smooth muscle of rats and to explore its mechanism.Methods PowerLab Signal Processing Instrument was used to observe the effects of puerarin on the activity of isolated intestinal smooth muscle of rats.Nitric oxide（NO） kits were used to detect the content of NO in isolated intestinal smooth muscle.Western blot was used to measure the expression of inducible nitric oxide synthase（iNOS） upregulated by puerarin.Results Puerarin（0.12,0.24,0.48 g/L） had significantly inhibited isolated intestinal smooth muscle of rats in a dose-dependent manner.Pretreatment of S-methylisothioure（SMT）,a specific iNOS inhibitor,the inhibitory effects of puerarin were attenuated.Following puerarin（0.12,0.24,0.48 g/L） administration,the amounts of NO and iNOS were decreased.Conclusions Puerarin could inhibit the contraction of isolated intestinal smooth muscle of rats mainly through the upregulation of iNOS expression and increasing NO release in intestine.

关 键 词：葛根素 离体肠道平滑肌 NO 诱导型NO合酶 

分 类 号：R286.11[医药卫生—中药学]