绿针假单胞菌HG28-5对辣椒疫病的抑制活性及其根际定殖特性的研究  

Inhibitory Activity of Pseudomonas chlororaphis HG28-5 Against Phytophthora Blight of Pepper and Its Root Colonization Characteristic

作　　者：申顺善[1] 赵玉华[1] 张利敬[1] 常淑娴[1] 王晶晶[1] 朴凤植[2] 

机构地区：[1]河南农业大学植物保护学院,新型农药创制与应用河南省重点实验室,郑州450002 [2]河南农业大学园艺学院,郑州450002

出　　处：《园艺学报》2013年第8期1574-1582,共9页Acta Horticulturae Sinica

基　　金：河南省重点科技攻关项目(092102110096;122102110042)

摘　　要：利用从小麦根系中分离筛选得到的对辣椒疫病有较高拮抗活性的生防细菌HG28-5菌株,通过平板对峙培养、产孢子囊和孢子萌发试验,检测其对辣椒疫霉菌的抑制作用;通过温室盆栽和小区试验,确认了对辣椒疫病的防治效果;以平板稀释培养法测定了根际定殖能力。结果表明:HG28-5显著抑制辣椒疫霉菌菌丝生长、游动孢子囊形成、游动孢子释放和休止孢子萌发;HG28-5对辣椒疫病具有明显的防治效果,在盆栽试验和小区试验中防治效果分别达到92.57%和79.96%;HG28-5在辣椒根际的定殖能力很强,用HG28-5处理辣椒种子,发芽胚根中其定殖密度为4.45×105cfu·cm-1,HG28-5处理盆栽辣椒根系,其定殖密度稳定维持在105cfu·g-1以上。利用形态特征、生理生化特性和16SrDNA序列分析,鉴定HG28-5为绿针假单胞菌。A promising biocontrol agent HG28-5,a strain with strong effect on Phytophthora blight of pepper,which selected from the root of healthy wheat（Triticum aestivum Linn.）. In vitro and in vivo experiments were used to determine its antifungal effect and root colonization ability. The results showed that,HG28-5 inhibited mycelial growth,germination of cystospores,and formation of zoospore and zoosporangia of Phytophthora capsici in vitro experiment;HG28-5 performed good control potential against Phytophthora blight of pepper in pot and vinyl house experiment,the control efficiencies were 92.57% and 79.96% respectively;HG28-5 successfully colonized on roots of pepper,the colonized density of HG28-5 was 4.45 × 10 5 cfu · cm -1 with Double Layer Filter paper method,in pot experiment,HG28-5persisted on root of pepper with a relative high population density which was more than 10 5 cfu · g -1 ; Based on the morphological,physiological characteristics and 16S rDNA sequence analysis,the strain HG28-5 was identified as Pseudomonas chlororaphis.

关 键 词：辣椒 疫病 生物防治 生防菌 绿针假单胞菌 

分 类 号：S641.3[农业科学—蔬菜学]