释药用丝素水凝胶的制备及体内外形态研究  

Study of the Preparation of Silk Fibroin Gel and Its Morphology as Drug Release Matrix in Vitro and in Vivo

作　　者：李青[1,2] 黄杰[3] 何飞[3] 贺庆梅[3] 曹传宝[1] 王松[1] 

机构地区：[1]北京理工大学材料科学与工程学院材料学,北京100081 [2]青岛中皓生物工程有限公司技术中心,青岛266071 [3]山东出入境检验检疫局,青岛266002

出　　处：《生物医学工程学杂志》2013年第4期794-797,811,共5页Journal of Biomedical Engineering

摘　　要：丝素(SF)/海藻酸钠(SA)水凝胶,可用作药物注射埋植的控释材料。本研究通过匀浆离心的方法制备猪心肌细胞提取物(PMM)并使其改性丝素凝胶材料,对比观察了SF、SF/SA、SF/SA/PMM不同组分的凝胶材料。结果表明SF/SA/PMM三元组分材料作为药物缓释材料可以缩短凝胶化时间、使凝胶形态更好地得到保持,同时使凝胶内部结构更加疏松,使孔壁变薄,更利于载药释药;病理切片结果表明其具有良好的生物相容性,并能增强骨髓间充质干细胞(BMSC)的生长活性。该结果对进一步开展药物的控释研究具有重要意义。Silk fibroin（SF）/sodium alginate（SA） hydrogels can be used as drug injection materials.Homogenate was prepared by centrifugation of the pig myocardial extracellular matrix（PMM） and its modification of SF gel material.This paper observes and compares the different components SF,SF/SA,SF/SA/PMM to illustrate the SF/SA/PMM ternary material as a drug delivery composition material.This ternary material can shorten the gel time,and can make the gel form to be maintained better.Meanwhile,it makes the internal structure of the gel looser so that the hole wall becomes thinner and more conducive to the drug release.In addition,it has good biocompatibility proved by pathological analysis,and is able to enhance the mesenchymal stem cells growth activity,which has great significance in carrying out drug control release.

关 键 词：心肌细胞基质 水凝胶 体内外 释药 

分 类 号：R318.08[医药卫生—生物医学工程]