务川黑牛STAT3基因启动子区SNPs与生长相关性状的关联分析  

Associations of Polymorphisms of Promoter Region of STAT3 Gene with Growth Traits in Wuchuan Black Cattle

作　　者：杨永强[1] 焦仁刚 龚俞 谢海强[1] 惠嫣婷[1] 张依裕[1] 肖超能[1] 刘若余[1] 

机构地区：[1]贵州大学动物科学学院/高原山地动物遗传育种与繁殖教育部重点实验室/贵州省动物遗传育种与繁殖重点实验室,贵阳550025 [2]贵州省畜牧技术推广站,贵阳550001

出　　处：《畜牧兽医学报》2013年第8期1205-1212,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：贵州省重大科技专项计划项目(黔科合重大专项字[2011]6009号)

摘　　要：为筛选STAT3基因启动子区SNPs及研究其对启动子功能元件和体尺性状的影响。选择品种差异较大的贵州荷斯坦奶牛和务川黑牛构建不同DNA池,直接测序筛选SNP位点,PCR-RFLP进行验证和基因分型,并利用114头务川黑牛个体分析SNPs位点与8个体尺性状的相关性。结果表明:STAT3基因5′调控区存在7个新SNPs位点,分别为:G-449A、C-341A、A-322G、C-246A、G-240A、C-225T、A-103T,提交SNP数据库得到登录号。生物信息学软件预测得到STAT3基因核心启动子区和CpG岛,SNP位点导致附近大量转录因子结合位点消失和新位点产生。多态性位点对转录因子结合位点有显著影响,但并不改变CpG岛大小。关联性分析结果表明,A-322G对务川黑牛体重有极显著影响(P<0.01),A等位基因为有利等位基因。与AA或GG纯合基因型相比,AG基因型个体有更出色的体重性状。A-322G可能作为影响肉牛生长性状的重要功能性SNP,STAT3基因可能作为肉牛选种选育的候选基因,研究结果为进一步确定STAT3基因功能奠定试验基础。The study aimed to screen the SNPs in the promoter region of STAT3 gene in cattle and analyze the effect of SNPs on function elements of promoter and growth traits.Two cattle breeds（Wuchuan Black cattle and Guizhou Holstein cow） that possessed significant difference in breeds property were selected to construct DNA pools,SNP sites were screened by direct sequencing subsequently.PCR-RFLP was used to be genotyping and a total of 114 Wuchuan Black cattle were used to study the associations of SNPs with 8 growth traits.Results showed that 7 new single nucleotide polymorphism（SNP） sites including G-449A,C-341A,A-322G,C-246A,G-240A,C-225T and A-103T,respectively were found in the 5＇ flanking region of STAT3 gene and then submitted the information of SNPs to NCBI to obtain accession numbers.Furthermore,bioinformatics tools were used to predict the core region of the promoter and CpG island.SNPs found in this study led to the disappearance of a large amount of transcription factors bingding sites and the appearance of new sites,it also showed that SNP mutation didn＇t affect the range of CpG island dramaticlly by using various softwares.Association analysis demonstrated that A-322G had highly significant effects on body weight（P0.01）,A allele was the favorable alleles.Moreover,animals with the genotype AG had the highest mean body weight than those with GG and AA genotypes,which suggested that A-322G was an important SNP marker affecting the cattle growth performance and STAT3 gene could be applied in marker-assisted selection for improving growth performance in cattle.This work could lay the experimental foundation for the identification of function of STAT3 gene in cattle.

关 键 词：STAT3 SNP 启动子 务川黑牛 生长性状 关联分析 

分 类 号：S823[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]