间情期水貂腔前卵泡卵母细胞的超微结构  

Ultrastructure Observation on Mink Preantral Follicle Oocytes during Dioestrus

作　　者：崔凯[1] 梁玉颖[1] 马泽芳[1] 

机构地区：[1]青岛农业大学动物科技学院,青岛266109

出　　处：《畜牧兽医学报》2013年第8期1317-1322,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：公益性行业(农业)科研专项资助(200903014)

摘　　要：为研究11月份间情期水貂卵母细胞的发育规律,探索其体外培养成熟的可能性,本研究利用透射电镜对间情期美国短毛黑水貂卵巢的腔前卵泡卵母细胞的超微结构进行观察。结果发现:在原始卵泡阶段,卵母细胞线粒体的嵴较少,高尔基体可见,脂滴大小不一,皮质颗粒分散在细胞核周围;初级卵泡阶段,线粒体数量增加,高尔基体趋于典型,脂滴分布均匀,皮质颗粒在质膜附近呈单层分布;次级卵泡阶段,线粒体嵴的数量增加,高尔基体在质膜下成群分布,脂滴增大、颜色变浅。11月份间情期水貂卵母细胞皮质颗粒和高尔基体的发育与发情期相比在分布位置上有所不同,而线粒体、内质网、脂滴等细胞器的发育与发情期的变化趋势基本一致,说明该时期水貂卵巢卵母细胞有体外培养成熟的可能。In order to study the developmental regularity of mink oocytes during dioestrus in November and to evaluate the in vitro maturing possibility of them,ultrastructure of ovaries preantral follicles of American short-hair black minks during dioestrus were observed by transmission electron microscope.Results showed that there were a less number of cristae at the primordial follicle stage.Golgi apparatus were visible.The size of lipid droplets was different,and cortical granules dispersed around the nucleus.At the primary follicular stage,the number of mitochondrion was increasing.Golgi apparatus tended to be typical.Lipid droplets distributed evenly.Cortical granules monolayer dispersed near the plasma membrane.At secondary follicle stage,the number of cristae was increasing.Flocks of Golgi apparatus distributed under the plasma membrane.The diameter of lipid droplets was increasing and the color of those was shallower.The results suggest that the distribution of cortical granules and Golgi apparatus in mink oocytes during dioestrus in November was different from that during estrus;while the developmental trend of mitochondria,endoplasmic reticulum,lipid droplets and other organelles during these two periods was the same.Therefore it is possible that mink oocytes obtained in November develop to mature in vitro.

关 键 词：水貂 间情期 腔前卵泡 卵母细胞 超微结构 

分 类 号：S814.8[农业科学—畜牧学]