染矽尘大鼠肺组织自噬现象观察  

Autophagy in lung tissue of rats exposed to silica dust

作　　者：陈适[1] 金玉兰[1] 姚三巧[1] 白玉萍[1] 范雪云[1] 徐应军[1] 袁聚祥[1] 

机构地区：[1]河北联合大学公共卫生学院河北省煤矿卫生与安全实验室,唐山063000

出　　处：《中华劳动卫生职业病杂志》2013年第8期607-610,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：基金项目：河北省自然科学基金资助项目（C2010001770）

摘　　要：目的研究游离SiO2粉尘刺激时，大鼠肺组织效应细胞在不同时间自噬活动的变化。方法选择60只体重220～230g Wistar大鼠，采用非气管暴露法建立大鼠矽肺模型，分别在染尘后1、7、14、21、28d各处死6只染尘组和6只对照组大鼠。取肺组织制作组织切片。采用苏木素-伊红（HE）染色法观察肺部炎症、纤维化等病理变化。用透射电子显微镜（TEM）观察肺组织切片中自噬前体、自噬体、自噬溶酶体的变化。结果染尘第1d，实验组和对照组中均可见较多自噬前体、自噬体；染尘第7d，实验组肺组织自噬体较对照组增多；染尘第14d，实验组自噬体较对照组减少；染尘第21和第28d实验组和对照组巨噬细胞胞浆中均观察到自噬溶酶体。实验组自噬溶酶体浊肿、膨胀、部分呈空泡化，第28d更加明显。结论游离SiO2粉尘可诱发大鼠肺组织自噬活动，不同染尘时间自噬活动强弱亦不同。Objective To investigate the autophagy of effector cells in lung tissue at different time points when rats were exposed to free SiO2 dust. Methods Sixty Wistar rats （220-230 g） were selected and allocated to experimental group （n=30） and control group （n=30）. In the experimental group, a rat silicosis model was established by infusing SiO2 suspension into the trachea of rats. Six rats in each group were sacrificed on days 1, 7, 14, 21, or 28 of dust exposure. Lung tissue samples were collected to prepare lung tissue sections. The pulmonary inflammation and fibrosis were observed by HE staining. The proautophagosome, autophagosome, and autophagolysosome in lung tissue sections were observed under a transmission electron microscope. Results On day 1 of dust exposure, many proautophagosomes and autophagosomes were seen in both experimental group and control group. On day 7 of dust exposure, the experimental group had more autophagosomes in lung tissue than the control group. On day 14 of dust exposure, the experimental group had fewer autophagosomes than the control group. On days 21 and 28, autophagolysosomes were seen in macrophage plasma in both experimental group and control group; the autophagolysosomes in experimental group showed cloudy swelling and expansion, and some were vacuolated, and these changes were more significant on day 28. Conclusion Free SiO2 dust can induce autophagy in the lung tissue of rats, with varying degrees at different time points of dust exposure.

关 键 词：矽肺模型 自噬 透射电镜 

分 类 号：R135[医药卫生—劳动卫生]