PTEN,Akt,TGF-β1蛋白在瘢痕疙瘩中的表达  

Expressions of PTEN, Akt and TGF-β1 protein in keloid

作　　者：郭亮[1] 徐凯[1] 章鑫[1] 黄巧玲[1] 桂思[1] 晏洪波[1] 李世荣[2] 

机构地区：[1]广州军区武汉总医院皮肤整形美容外科,湖北武汉430070 [2]第三军医大学西南整形美容专科医院

出　　处：《中国美容整形外科杂志》2013年第8期505-507,共3页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：湖北省自然科学基金（2012FFB06808）

摘　　要：目的 探讨瘢痕疙瘩组织中TGF-β1、第十号染色体同源丢失性磷酸酶基因（phosphatase and tensin homolog deleted on Chromosome 10,PTEN）和蛋白激酶（Akt）的表达.方法 采用免疫组化SP法检测TGF-β1、PTEN和p-Akt在28例瘢痕疙瘩及20例正常皮肤组织中的表达水平.结果 瘢痕疙瘩组织中,TGF-β1、p-Akt蛋白阳性表达率分别为43.94±7.59、44.95±6.65,均高于正常皮肤组（P ＜0.01）;PTEN蛋白阳性表达率为6.53±1.45,低于正常皮肤组（P ＜0.01）.PTEN的表达与p-Akt、TGF-β1的表达强度成负相关（r=-0.6437,-0.376）,TGF-β1与p-Akt表达成正相关（r =0.804）.结论 PTEN、Akt及TGF-β1在瘢痕疙瘩的发生发展中均起着重要的调控作用,PTEN/Akt信号传导通路可能通过调控TGF-β1参与瘢痕疙瘩的形成.Objective To investigate the expressions of TGF-β1, p-Akt and PTEN proteins in keloid. Methods SP immunohistochemical method was used to detect the expressions of TGF-β1, p-Akt and PTEN proteins in 28 cases of pathologic sear and 20 cases of normal skin tissue. Results The positive rates of TGF- β1 and p-Akt proteins in 28 cases of keloid were 43.94 ±7.59 and 44.95 ±6.65, were higher respectively compared with the control groups （ P〈0.01 ） ;The rate of PTEN proteins in keloid group was 6.53 ±1.45, significantly lower than the normal skin group （ P〈0.01 ） . A negative correlation was found between the expression of PTEN and p-Akt or TGF-β1 proteins （r = -0. 6437, -0. 376） in keloid, and there was a highly positive correlation between TGF-β1 and p-Akt protein expression （ r = 0. 804）. Conclusion PTEN, Akt and TGF-β1 protein might be involved in the formation of keloid. PTEN/Akt signaling pathway may regulate the information of keloid through the activation of TGF-β1.

关 键 词：瘢痕疙瘩 PTEN P-AKT TGF-Β1 

分 类 号：R619.6[医药卫生—外科学]