栀子苷对LPS诱导的小鼠乳腺炎的抑制效果  

Inhibited effects of gardenoside on the LPS-induced mice mastitis

作　　者：孙勇[1] 李德鹏[1] 张雯[1] 刘志成[1] 李丰阳[1] 梁德洁[1] 杨正涛[1] 曹永国[1] 张乃生[1] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062

出　　处：《中国兽医科学》2013年第8期876-880,共5页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31272622);教育部博士点基金项目(20110061130010)

摘　　要：选取雌、雄小鼠各24只合笼,随机将其分为6组,用LPS灌注乳池,建立小鼠乳腺炎模型。以不同浓度的栀子苷(25、50、100mg/kg)及地塞米松给药后第24小时采取乳腺组织,研究栀子苷对小鼠乳腺炎组织病理学及髓过氧化物酶(MPO)、TNF-α、IL-6、IL-1β表达的影响。结果显示,与LPS组相比,栀子苷能抑制乳腺组织的病理损伤,且其低、中、高剂量组MPO的表达量依次降低。与对照组相比,LPS组中TNF-α、IL-6、IL-1β的表达量显著升高,且差异极显著(P<0.01)。与LPS组相比,栀子苷低、中、高剂量组和地塞米松组TNF-α、IL-6、IL-1β的表达量明显降低,且差异极显著(P<0.01)。结果表明,栀子苷对小鼠乳腺炎的发生具有抑制效果,且在一定范围内浓度越高抑制效果越强,具有量效关系。To investigate the protective effects of gardenoside on the mice against mastitis,48 male and female mice were paired and randomly divided into 6 groups.Mouse mastitis models were established by pouring LPS into mammary gland after farrowing.Female mice with mastitis were treated with different concentrations of gardenoside（25,50 and 100 mg/kg） and dexamethasone,respectively. Mammary glands of the mice were collected at 24 h after the treatment to study the effects of gardenoside on the histopathology and the expression of myeloperoxidase（MPO）,TNF-α,IL-6 and IL-1β. In result,the expression levels of TNF-α,IL-6 and IL-1β were significantly higher in the LPS group than the normal group,indicating the model of mastitis was established successfully in this study.Mammary gland pathological damages were significantly inhibited by gardenoside treatment compared with the LPS group（P0.01）.MPO expression levels were decreased with the treatment of low,medium and high doses of gardenoside,and the expression levels of TNF-α,IL-6 and IL-1β were significantly lower in the gardenoside group than the dexamethasone group.These results indicated that gardenoside has inhibitory effect on mice mastitis,and the inhibitory effect exhibits a dose-dependent manner.

关 键 词：栀子苷 乳腺炎 小鼠 髓过氧化物酶 细胞因子 

分 类 号：S853.75[农业科学—临床兽医学]