反硝化细菌生物反应器去除地浸采铀矿山退役采区地下水中NO_3^--N的试验研究  

Removal of NO_3^--N From Polluted Groundwater in Decommissioned Mining Area in an In-situ Leach Uranium Mine by Denitrifying Bacteria Bioreactor

作　　者：王清良[1,2] 胡南[1] 丁德馨[1] 张国奇[1] 胡鄂明[1] 张洪灿[1] 阳奕汉 蒋晓辉 

机构地区：[1]南华大学铀矿冶生物技术国防重点学科实验室,湖南衡阳421001 [2]中南大学资源加工与生物工程学院,湖南长沙410083 [3]新疆中核天山铀业有限公司,新疆伊宁835000

出　　处：《原子能科学技术》2013年第8期1300-1306,共7页Atomic Energy Science and Technology

基　　金：国家自然科学基金资助项目(50774047;51154003);湖南省科技厅重点项目资助(2010GK2025);湖南省教育厅重点项目资助(10A103);湖南省教育厅项目资助(10C1134)

摘　　要：地浸采铀矿山退役采区地下水的NO3--N污染是一备受关注的问题。本文通过对取自某地浸采铀矿山退役采区的污泥进行驯化,得到了能去除地浸采铀矿山退役采区污染地下水中NO3--N的反硝化细菌,自行设计了上流式固定床反硝化细菌生物反应器,研究了pH值、C/N比和水力停留时间(HRT)对反硝化细菌生物反应器去除地浸采铀矿山退役采区污染地下水中NO3--N的影响。研究结果表明:当进液pH值为6.50、NO3--N浓度为1 000mg/L、HRT为2.3h时,NO3--N的去除率和去除速率分别达97%和388mg/(h.L),生物反应器处理废水的能力达0.35m3/(h.m3);当进液NO3--N浓度为550mg/L、HRT为1.4h时,NO3--N的去除率和去除速率分别达96%和368mg/(h.L),生物反应器处理废水的能力达0.62m3/(h.m3);反硝化细菌生物反应器适宜的运行条件是pH值为5.00～8.00,C/N比为0.6～0.8。The pollution of groundwater by NO3- in the decommissioned mining area in an i,rsitu leach uranium mine is being paid more and more attention. The denitrifying bacteria by domesticating the sludge taken from the decommissioned mining area in an in-situ leach uranium mine in North West of China were obtained. An up-flow fixed-bed denitrifying bacteria bioreactor was designed. The effects of pH, the ratio of carbon to nitrogen and HRT on the removal of NO3-N from the polluted groundwater in the decommissioned mining area in the in-situ leach uranium mine by the denitrifying bacteria bioreactor were investigated. The results show that when pH, the concentration of NO;-N and HRT are set to 6.50, 1 000 mg/L and 2.3 h, respectively, the removal of the NO3-N amounts to 97%, the N（）3-N degradation rate is 388 mg/（h ; L） and the capacity of the bioreactor is 0.35 m3/（h ; m3）. When pH, the concentration of NO3-N and HRT are set to 6.50, 550 mg/L and 1.4 h, respectively, the removal of the NO3-N amounts to 96% and the capacity of the bioreactor is 0.62 m3/（h m3）. The bioreactor should work on the operation conditions that pH is 5.00-8.00 and the ratio of carbon to nitrogen is 0.6-0.8.

关 键 词：反硝化细菌生物反应器 微生物修复 地浸采铀 污染地下水 NO3--N 

分 类 号：X52[环境科学与工程—环境工程]