内源性蛋白质分子成像的研究进展（英文）  

Molecular Imaging Using Endogenous Cellular Proteins

作　　者：周进元[1,2] 洪晓华[1,3] 

机构地区：[1]约翰霍普金斯大学放射系磁共振研究部,美国马里兰州巴尔的摩市21287 [2]F.M.Kirby脑功能成像研究中心,美国马里兰州巴尔的摩市21205 [3]华中科技大学同济医学院附属协和医院肿瘤科,湖北武汉430020

出　　处：《波谱学杂志》2013年第3期307-321,共15页Chinese Journal of Magnetic Resonance

基　　金：National Natural Science Foundation of China(81128006);US National Institutes of Health(R01EB009731,R21EB015555)

摘　　要：酰氨质子转移(amide proton transfer,APT)成像是一种新的分子MRI技术,它可用来测量组织中内源性蛋白质.理论上,APT-MRI信号强度主要取决于游离蛋白质的酰氨质子浓度以及交换速度,而酰氨质子交换速度与组织pH有关.因此,APTMRI技术已经被用于无创性中风pH成像(通常pH降低)和肿瘤蛋白质含量成像(通常蛋白质量提高).近期对大鼠的实验表明,APT-MRI技术可用来区分放射性坏死和胶质瘤.该综述文章简要地介绍了APT成像的基本原理以及它在动物模型与临床中风和肿瘤成像中的应用.Amide proton transfer （APT） imaging is a novel molecular MRI technique that generates image contrast based on endogenous cellular proteins in tissue. Theoretically, the APT- MRI signal depends primarily on the mobile amide proton concentration and amide proton exchange rates （which are related to tissue pH）. The APT technique has been used for non-invasive pH imaging in stroke （where pH drops） and protein content imaging in tumor （where many proteins are overexpressed）. It has been demonstrated recently in animal models that the APT- MRI signal is also a unique imaging biomarker to distinguish between radiation necrosis and active tumor. In this paper, we will briefly introduce the basic principles of APT imaging and review its applications in stroke and brain tumor studies in animal models and patients.

关 键 词：酰氨质子转移 pH成像 分子成像 中风 脑瘤 

分 类 号：O482.53[理学—固体物理]