春季保护地小型西瓜苗期枯萎病和叶枯病初步鉴定  

Identification of Fusarium Wilt and Leaf Blight at Seedling Stage of Mini-watermelon under Protected Fields in Spring

作　　者：戴照义[1] 吴金平[1] 王运强[1] 郭凤领[1] 韦向阳[1] 

机构地区：[1]湖北省农业科学院经济作物研究所,武汉430064

出　　处：《长江蔬菜》2013年第16期68-70,共3页Journal of Changjiang Vegetables

基　　金：国家现代农业产业技术体系专项资金资助(CARS-26-34)

摘　　要：随着春保护地西瓜种植面积的扩大和工厂化育苗的发展,西瓜苗期病害也越来越严重,有些常见于生长期的病害,现在在苗期也有较重发生。对湖北省宜城市流水镇育苗场采集的2份西瓜苗期病样,进行分离、纯化并回接病原菌,然后利用PCR扩增真菌保守的ITS序列,初步确定所采集病样1-a的致病菌为枯萎病病原菌[Fusarium oxysporum Schl.f.sp.niveum(E.F.Smith)Snyber et Hansen],病样1-b的致病菌为叶枯病病原菌[Alternaria cucumerina(Ell.et Ev.)Elliott]。本研究利用分子鉴定手段,避免了传统分类方法的繁琐、耗时和结果不稳定性等缺点。With the expansion of planting area and the development of the industrial watermelon seedling production in protected fields, the seedling diseases were becoming more and more seriously, and some common diseases of growing period occurred badly at the seedling stage. In the paper, firstly, we collected two samples with seedling diseases, and separated, purified and inoculated the pathogenic fungi. Secondly, we amplified the conserved ITS sequences of the fungi. Thirdly, we preliminary determinated the pathogenic fungus of sample 1-a and sample 1-b was Fusarium wilt [Fusarium oxyspomm Schl. f. sp. niveum （E. F. Smith） Snyber et Hansen] and leaf blight [Alternaria cucumenna （Ell. et Ev.） Elliott] respectively. In the paper, we used molecular identification method, effectively avoiding the application of tedious, time- consuming and unstable traditional classification methods.

关 键 词：西瓜 枯萎病 叶枯病 苗期病害 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]